202280. lajstromszámú szabadalom • Eljárás funkcionális humán urokináz fehérjék előállítására
1561 1621 1681 1741 1801 1861 1921 1981 2041 2101 2161 2221 2281 HU 202280 B Int Cl5 C 12 N 15/58 C 12 N 15/70 C 12 N 15/85 Hl MnlIHaelHaelIIBstNIMnlIAvalIHi nfI CAGACCCTCTGGCCAGGATGGAGGGGTGGTCCTGACTCAACATGTTACTGACCAGCAACT GTCTGGGAGACCGGTCCTACCTCCCCACCAGGACTGAGTTGTACAATGACTGGTCGTTGA BalIEcoRII Sau96 PstI TGTCTTTTTCTGGACTGAAGCCTGCAGGAGTTAAAAAGGGCAGGGCATCTCCTGTGCATG ACAGAAAAAGACCTGACTTCGGACGTCCT CAATTTTTCCCGTCCCGT AGAGGACACGT AC Taql Alul Hpall MnlIHaelII GGCTCGAAGGGAGAGCCAGCTCCCCCGACCGGTGGGCATTTGTGAGGCCCATGGTTGAGA CCGAGCTTCCCTCTCGGTCGAGGGGGCTGGCCACCCGTAAACACTCCGGGTACCAACTCT Sau96NcoI XmnI BbvPvuIIOdel Mnll Alul AATGAATAATTTCCCAATTAGGAAGTGTAAGCAGCTGAGGTCTCTTGAGGGAGCTTAGCC TT ACTT ATT AAAGGGTT AATCCTTCACATTCGT CGACTCCAGAGAACTCCCTCGAATCGG Fnu4HIAluIMnlI Ddel Fnu4HI AATGTGGGAGCAGCGGTTTGGGGAGCAGAGACACTAACGACTTCAGGGCAGGGCTCTGAT TTACACCCTCGTCGCCAAACCCCTCGTCTCTGTGATTGCTGAAGTCCCGTCCCGAGACTA Bbv ATTCCATGAATGTATCAGGAAATATATATGTGTGTGTATGTTTGCACACTTGTTGTGTGG T AAGGT ACTT AC AT AGTCCTTT AT ATAT AC AC AC AC AT AC AAACGT GTGA AC A AC AC AC C Alul GCTGTGAGTGTAAGTGTGAGTAAGAGCTGGTGTCTGATTGTTAAGTCTAAATATTTCCTT CGACACTCACATTCACACTCATTCTCGACCACAGACTAACAATTCAGATTTATAAAGGAA SfaNI Mill E AAACT GTGT GGACTGTGAT GCCACACAGAGTGGTCTTTCTGGAGAGG TT AT AGGTCACTC TTTGACACACCTGACACTACGGTGTGTCTCACCAGAAAGACCTCTCCAATATCCAGTGAG B coRIISau96ta1ISau96 EcoRII Rsal PstIFnu4HI CTGGGGCCTCTTGGGTCCCCCACGTGACAGTGCCTGGGAATGTACTTATTCTGCAGCATG GACCCCGGAGAACCCAGGGGGTGCAC TGTCACGGACCC TTACATGAATAAGACGT CGTAC stNIHaelll Avail BstNI Bbv Ddel HaelHaell AC CTGTGAC CAGCAC TGTC TCAGTTTCAC TTTCACATAGAT GTCCCTTTCTTGGCCAGTT T GGACACTGGTCGTGACAGAGTCAAAGTGAAAGT GTATCT ACAGGGAAAGAACC GGTCAA Ball I Mnll Hphl Sau96 ATCCCTTCCTTTTAGCCTAGTTCATCCAATCCTCACTGGGTGGGGTGAGGACCACTCCTT TAGGGAAGGAAAAT CGGAT CAAGT AGGTT AGGAGT GACCCACCCCACTCCTGGTGAGGAA MnlIAvalI Be ACACTGAATATTTAT ATTTCACT ATTTTT ATTT ATATTTTTGTAATTTT AAATAAAAGTG TGTGACTTAT AAAT AT AAAGTGAT AAAAAT AAAT AT AAAAACATTAAAATTTATTTTCAC S 1IOpnl AT CAAT AAAATGTGATTTTT CTGA TAGTTATTTTACACTAAAAAGACT au3A 3C.ábra
