202280. lajstromszámú szabadalom • Eljárás funkcionális humán urokináz fehérjék előállítására

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Int Cl5

HU 202280 B C 12 N 15/58 C 12 K 15/70 C 12 N 15/85

BbvHaell Thal MnlI Fnu4HIHaeIII MnlIFnu4HI Hael GTCCCCGCAGCGCCGTCGCGCCCTCCTGCCGCAGGCCACCGAGGCCGCCGCCGTCTAGCG CAGGGGCGTCGCGGCAGCGCGGGAGGACGGCGTCCGGTGGCTCCGGCGGCGGCAGATCGC Fnu4HIHhaI Hhal HaelBglI HaeIIIFnu4HI Hha

I Mnll BglI Thai HgalEcoRII

CCCCGACCTCGCCACCATGAGAGCCCTGCTGGCGCGCCTGCTTCTCTGCGTCCTGGTCGT GGGGCTGGAGCGGTGGTACTCTCGGGACGACCGCGCGGACGAAGAGACGCAGGACCAGCA I HhalHhal BstNl

HinfI Bbv Taql

GAGCGACTCCAAAGGCAGCAAT GAACTTCAT CAAGT TCCATCGAACTGT GACTGTCTAAA CTCGCTGAGGTTTCCGTCGTTACTTGAAGTAGTTCAAGGTAGCTTGACACTGACAGATTT Fnu4HI

Mnll Rsal

TGGAGGAACATGTGTGTCCAACAAGTACTTCTCCAACATTCACTGGTGCAACTGCCCAAA

ACCTCCTTGTACACACAGGTTGTTCATGAAGAGGTTGTAAGTGACCACGTTGACGGGTTT

MnlIBbv Mnll

GAAATTCGGAGGGCAGCACTGTGAAATAGATAAGTCAAAAACCTGC TATGAGGGGAATGG CTTTAAGCCTCCCGTCGTGACACTTTATCTATTCAGTTTTTGGACGATACTCCCCTTACC Fnu4HI

Mnll Hael Ncol HaelIIHpalISau96BstNI

TCACTTTTACCGAGGAAAGGCCAGCACTGACACCATGGGCCGGCCCTGCCTGCCCTGGAA AGTGAAAATGGCTCCTTTCCGGTCGTGACTGTGGTACCCGGCCGGGACGGACGGGACCTT Hael11 Sau96NaeIHaeIII EcoRII

Rsal SfaNIXhoIISau3AMboIIAluISa CTCTGCCACTGTCCTTCAGCAAACGTACCATGCCCACAGATCTGATGCTCTTCAGCTGGG GAGACGGTGACAGGAAGTCGTTTGCATGGTACGGGTGTCTAGACTACGAGAAGTCGACCC

BglIlDpnI PvuII Ha

u96EcoRII PstI Hpall EcoRII

CCTGGGGAAACATAATTACTGCAGGAACCCAGACAACCGGAGGCGACCCTGGTGCTATGT GGACCCCTTTGTATTAATGACGTCCTTGGGTCTGTTGGCCTCCGCTGGGACCACGATACA el I IBstNI Mnll BstNl

Sau96 Fnu4HI MstI

GCAGGTGGGCCTAAAGCCGCTTGTCCAAGAGTGCATGGTGCATGACTGCGCAGATGGAAA CGTCCACCCGGATTTCGGCGAACAGGTTCTCACGTACCACGTACTGACGCGTCTACCTTT Hael II Hhal

Mnll tall MboII HaelHaelIIHinfItalISa

AAAGCCCTCCTCTCCTCCAGAAGAATTAAAATTTCAGTGTGGCCAAAAGACTCTGAGGCC TTTCGGGAGGAGAGGAGGTCTTCTTAATTTTAAAGTCACACCGGTTTTCTGAGACTCCGG Mnll Ball DdelHael

u96 EcoRI Taql BstNl Fnu4H

CCGCTTTAAGATTATTGGGGGAGAATTCACCACCATCGAGAACCAGCCCTGGTTTGCGGC GGCGAAATTCTAATAACCCCCTCTTAAGTGGTGGTAGCTCTTGGTCGGGACCAAACGCCG II Hphl EcoRII Hae

I tall Hpall Hphl MnlIBbv

CATCT ACAGGAGGCAC CGGGGGGGCTCTGT CAC CTACGT GTGT GGAGGCAGCCTCATCAG GT AGAT GT CCTCCGT GGCCCCCCCGAGACAGTGGAT GCACACACCTCCGT CG6AGTAGTC III Ne iI Fnu4HIMnlI

HphIOpnlHaelI tall

CC CTTGCT GGGT GAT CAGCGCCACACACTGCTT CATTGATTACCCAAAGAAGGAGGAC TA GGGAACGACCCACTAGTCGCGGTGTGTGACGAAGTAACTAATGGGTTTCTTCCTCCTGAT BclISau3AHhaI

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