201093. lajstromszámú szabadalom • Eljárás szomatotropin szolubilizálására és kinyerésére

5

Hü 201093 B

1. Táblázat

6

Reprezentatív szomatotropinok aminosav-szekvenciája

!Ö —

BGH Phe-Pro-Ala-Met-Ser-Leu-Ser-Gly-Leu-Phe-Ala-Asn-Ala-Val-Leu-PGH--------------------------Pro--------------Ser— ----------—-—-—-------------------OGH------------------------------------------------------------------------------------------------------ÁGH---------------------------Pro----------------Asn------------------------------------------------HGH--------------Thr-Ile-Pro----------------Arg--------------Asp--------------Met---------20 30

BGH Arg-Ala-Gln-Hi6-Leu-His-Gln-Leu-Ala-Ala-Asp-Thr-Phe-Lys-Glu-PGH------------------------------------------------------------------------------Tyr----------------OGH----------------------------------------------------------------------------------------------------AGH----------------------------------------Leu---------------------Gin--------Tyr---------------HGH--------------His-Arg----------------------------------Phe--------------Tyr-Gln-----------40

BGH Phe-Glu-Arg-Thr-Tyr-Ile-Pro-Glu-Gly-Gln-Arg-Tyr-Ser—X—Ile-PGH----------------------Ala-------------------------------------------------------------------------OGH----------------------------------------------------------------------------------------------------AGH-----------------------------------------------------A6p---------------------Thr--------Asn----HGH--------------Glu-Ala---------------------Lys-Glu--------Lys--------------Phe-Leu 50 60

BGH Gln-Asn-Thr-Gln-Val-Ala-Phe-Cys-Phe-Ser-Glu-Thr-Ile-Pro-Ala-PGH---------------Ala-------Ala-------------------------------------------------------------------OGH-------------------------------------------------------------------------------------------------AGH Lys---------Ser--------Ala----------------------Tyr--------------------------------------HGH--------------Pro--------Thr-Ser-Leu-----------------------------Ser--------------Thr-70

BGH Pro-Thr-Gly-Lys-Asn-Glu-Ala-Gln-Gln-Lys-Ser-Asp-Leu-Glu-Leu-PGH----------------------------Asp---------------------------Arg----------------Val-------------OGH-----------------------------------------------------------------------------------------------------AGH----------------------------Asp-Asp------------------------------------------Met-Gly-------HGH-------Ser-Asn-Arg-Glu--------Thr-----------------------------Asn--------Gin-------80 90

BGH Leu-Arg-Ile-Ser-Leu-Leu-Leu-Ile-Gln-Ser-Trp-Leu-Gly-Pro-Leu-PGH---------------Phe---------------------------------------------------------------------------Val-OGH------------------------------------------------------------------------------------------------------AGH--------------Phe--------------Val--------------------------------------------Thr--------Val-HGH -------------------—————————————---------—------Glu——Val—

100

BGH Gin-Phe-Leu-Ser-Arg-Val-Phe-Thr-Asn-Ser-Leu-Val-Phe-Gly-Thr-PGH------------------------------------------------------------------------------------------------------OGH----------------------------------------------------------------------------------------------------—

AGH-------Tyr----------------Lys-----------------------------Asn---------------------------------HGH----------------------Arg-Ser---------------Ala------------------------------Tyr--------Ala-110 120 BGH Ser-Asp-Arg—X—Val-Tyr-Glu-Lys-Leu-Lys-Asp-Leu-Glu-Glu-Gly-PGH------------------------------------------------------------------------------------------------------OGH------------------------------------------------------------------------------------------------------AGH-----------------------------------Phe--------------------------------------------------------------HGH-------Asn-Ser-Asp--------------Asp-Leu-------------------------------------------------130

BGH Ile-Leu-Ala-Leu-Met-Arg-Glu-Leu-Glu-Asp-Gly-Thr-Pro-Arg-Ala-PGH-------Gin— --------------------------------------------------------Ser----------------------OGH------—----------——----——---------------------------------—Val-------——------—-----AGH-------Gin---------------------------------------------------------Arg-Ser--------------Gly-HGH-------Gln-Thr--------------Gly-Arg------------------------------Ser--------------Thr-Gin------Gln-Thr-Gly-Arg-Gly—Thr-5