200795. lajstromszámú szabadalom • Eljárás többszörös horgokat tartalmazó plazminogén aktivátorok előállítására
HU 200795 B Int Cl5 C 12 N 15/58 A 61 K 37/54 Az 1/b ábra szerinti hármas-horog PA 1 11 21 31 41 31 GGATCCAGCAATCATGGATGCAATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTGTGG MetAspAlaMetLysArgGlyLeuCysCysValLeuLeuLeuCysGly 61 71 81 91 101 111 AGCAGTCTTCGTTTCGCCCAGCCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAGATC AlaValPheValSerProSerGlnGluIleHisAlaArgPheArgArgGlyAlaArgSer 121 131 141 151 161 171 TTACCAAGTGATCTGCAGAGATGAAAAAACGCAGATGAT AT ACCAGCAACAT CAGTCAT G TyrGlnValIleCysArgAspGluLysThrGlnMetlleTyrGlnGlnHisGlnSerTrp 181 191 201 211 221 231 GCTGCGCCCTGTGCTCAGAAGCAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAGGGC LeuArgProValLeuArgSerAsnArgValGluTyrCysTrpCysAsnSerGlyArgAla 241 251 261 271 281 291 ACAGTGCCACTCAGTGCCTGTCAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGGCAG GlnCysHisSerValProValLysSerCysSerGluProArgCysPheAsnGlyGlyThr 301 311 321 331 341 351 CTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGCTGG CysGlnGlnAlaLeuTyrPheSerAspPheValCysGlnCysProGluGlyPheAlaGly 361 371 381 391 401 411 GAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTATGAGGGGAATGGTCACTTTTACCG LysCysCysGluIleAspThrArgAlaThrCysTyrGluGlyAsnGlyHisPheTyrArg 'so---------------------------------------------421 431 441 451. 461 471 AGGAAAGGCCAGCACTGACACCATGGGCCGGCCCTGCCTGCCCTGGAACTCTGCCACTGT GlyLysAIaSerThrAspThrMetGlyArgProCysLeuProTrpAsnSerAlaThrVal 541 551 561 571 581 591 TAATTACTGCAGGAACCCAGACAACCGGAGGCGACCCTGGTGCTATGTGCAGGTGGGCCT AsnTyrCysArgAsnProAspAsnArgArgArgProTrpCysTyrValGlnValGlyLeu 601 611 621 631 641 651 AAAGCCGCTTGTCCAAGAGTGCATGGTGCATGACTGCAGCGAGGGCAACTCCGACTGCTA LysProLeuValGlnGluCysMetValhiisAspCysSerGluGlyAsnSerAspCysT yr I ......... 3 131 92 661 671 681 691 701 711 CGAGGACCAGGGCATCAGCTACAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTG GluAspGlnGlylleSerTyrArgGlyThrTrpSerThrAlaGluSerGlyAlaGluCys 721 731 741 751 761 771 CACCAACTGGAACAGCAGCGCGTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGACGC ThrAsnTrpAsnSerSerAlaLeuAlaGlnLysProTyrSerGlyArgArgProAspAla 781 791 801 811 821 831 CATCAGGCTGGGCCTGGGGAACCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCC IleArgLeuGlyLeuGlyAsnHisAsnTyrCysArgAsnProAspArgAspSerLysPro 841 851 861 871 881 891 CTGGTGCTACGTCTTTAAGGCGGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTG TrpCysTyrValPheLysAlaGlyLysTyrSerSerGluPheCysSerThrProAlaCys tPA szignál peptid 30 aminosav UK horog aa 50-131 10. ábra
