200366. lajstromszámú szabadalom • Eljárás módosított higromicin rezisztencia gén előállítására
1 HU 200366 B 2 ccc 1 1 1 CAT 1 1 1 GTG i t t TAT 1 1 1 CAC 1 1 1 TGG 1 1 1 CAA 1 1 1 1 1 1 GGG 1 1 1 GTA 1 1 1 CAC 1 1 1 ATA 1 1 t GTG 1 I 1 ACC 1 I 1 GTT GTC 1 1 1 AGT 1 1 1 GCG 1 1 1 TCC 1 1 1 GTC 1 1 1 GCG 1 I 1 CAG 1 1 1 1 1 I CAG 1 1 1 TCA 1 1 1 CGC 1 i ! AGG 1 1 1 CAG 1 1 1 CGC 1 I 1 GTC CTT 1 1 1 TGG 1 1 1 GCC 1 1 i GAG 1 1 1 GAC 1 1 1 TGC 1 1 1 CCC 1 1 1 i 1 1 GAA 1 1 ! ACC 1 1 1 CGG 1 1 1 CTC 1 1 1 CTG I 1 1 ACG 1 1 1 GGG CAC 1 1 1 GCG 1 1 1 GAT 1 1 1 TTC 1 1 1 GGC l i I TCC 1 1 I AAC 1 i 1 1 1 1 GTG 1 1 1 CGC 1 1 ! CTA 1 1 1 AAG 1 1 1 CCG 1 1 t AGG 1 i 1 TTG AAT 1 1 1 GGC 1 1 1 CGC 1 i 1 ATA 1 1 1 ACA 1 1 1 GCG 1 1 1 GTC 1 1 t 1 1 1 TTA 1 1 1 CCG 1 I 1 GCG 1 1 1 TAT 1 1 1 TGT 1 1 1 CGC 1 1 1 CAG ATG 1 1 1 TTC 1 1 1 GGG 1 1 1 GAT 1 1 t TCC 1 1 1 CAA 1 1 1 TAC 1 1 1 1 1 1 TAC 1 1 t AAG 1 1 1 CCC 1 1 1 CTA 1 1 1 AGG 1 1 1 GTT 1 1 1 ATG TTC 1 1 1 TGG 1 1 1 AGG 1 1 ! CCG 1 1 1 TGG 1 1 1 TTG 1 t 1 GCT t 1 1 1 1 1 AAG 1 I 1 ACC 1 1 1 TCC 1 1 1 GGC 1 1 1 ACC 1 1 1 AAC 1 1 1 CGA CGC 1 i 1 TAC 1 1 1 TTC 1 1 1 GAG 1 1 1 CGG 1 i 1 AGG t 1 I CAT 1 1 1 1 i I GCG I 1 1 ATG 1 1 1 AAG 1 1 1 CTC 1 1 1 GCC 1 1 1 TCC 1 1 1 GTA CCG i i i CGG 1 1 1 CTC 1 1 I CGG 1 t 1 GCG 1 1 1 TAT 1 1 1 ATG 1 1 f GGC 1 1 1 GCC 1 1 I GAG 1 1 1 GCC 1 1 1 CGC 1 1 1 ATA TAC CAA i i i CTC 1 1 1 TAT 1 1 1 CAG 1 t t AGC 1 ! 1 TTG 1 1 1 GTT 1 1 1 J 1 1 GTT 1 I 1 GAG 1 1 1 ATA 1 t t GTC 1 1 1 TCG 1 ] 1 AAC ! i 1 CAA GCA i i i GCT 1 1 1 TGG 1 1 i GCG 1 f 1 CAG 1 1 1 GGT f 1 l CGA 1 1 1 1 i 1 CGT 1 1 1 CGA I I I ACC 1 I 1 CGC i 1 1 GTC ( 1 1 CCA I 1 1 GCT TCC 1 I I GGA I 1 I GCC I ( I GGG ( ( t ACT < ( f GTC ( { ( GGG ( I ( I 1 1 AGG 1 1 1 CCT 1 1 1 CGG 1 1 1 CCC 1 1 1 TGA 1 1 I CAG 1 1 I CCC AGA 1 1 1 AGC 1 1 1 GCG 1 1 1 GCC 1 1 I GTC 1 1 1 TGG 1 1 1 ACC 1 1 I 1 1 1 TCT 1 1 1 TCG 1 1 1 CGC 1 1 1 CGG 1 1 1 CAG 1 1 1 ACC 1 1 1 TGG CTC t 1 1 GCC 1 1 1 GAT 1 1 1 AGT 1 1 1 GGA 1 1 t AAC 1 1 1 CGA i 1 I 1 1 1 GAG 1 1 I CGG 1 1 1 CTA 1 1 ! TCA 1 1 1 CCT 1 1 1 TTG 1 1 1 GCT AGG 1 t 1 GCA t 1 1 AAG 1 1 1 GAA 1 ( 1 TAG 1 1 1 1 1 1 TCC 1 1 1 CGT 1 1 1 TTC 1 1 i CTT 1 1 1 ATC ahol: A dezoxi-adenil-, G dezoxi-guanidil-, C dezoxi-citizil- és T timidilcsoport; 65 ACT GTG ATG GAC GAC ACC 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 TGA CAC TAC CTG CTG TGG GCT 1 1 1 CTC 1 1 1 GAT 1 1 i GAG 1 1 1 CTG 1 t 1 ATG 1 1 1 1 1 1 CGA 1 1 1 GAG 1 1 I CTA 1 1 1 CTC 1 1 1 GAC 1 1 1 TAC GAA 1 1 1 GTC 1 1 1 CGG 1 1 1 CAC 1 1 1 CTC GTG 1 1 1 1 1 1 CTT 1 1 1 CAG 1 1 1 GCC 1 1 1 GTG 1 1 i GAG 1 1 1 CAC AAT i i i GTC 1 1 1 CTG 1 1 1 ACG 1 1 1 GAC 1 1 1 AAC 1 1 1 i I 1 TTA 1 1 1 CAG 1 1 1 GAC 1 1 1 TGC 1 1 1 CTG 1 1 1 TTG ATT i i i GAC 1 1 1 TGG 1 1 I AGC 1 1 1 GAG 1 1 1 GCG t 1 1 i i i TAA 1 1 ! CTG 1 1 1 ACC 1 1 i TCG 1 1 1 CTC i 1 1 CGC GAG i i i GTC 1 1 i GCC 1 1 1 AAC 1 1 1 ATC 1 1 1 TTC 1 1 1 i i 1 CTC 1 1 1 CAG 1 1 1 CGG 1 I 1 TTG 1 1 1 TAG 1 1 ! AAG TGT i i i ATG 1 1 1 GAG 1 1 1 CAG 1 1 1 CAG 1 1 1 ACG 1 1 1 i i i ACA l l 1 TAC I 1 1 CTC 1 1 t GTC 1 1 1 GTC 1 1 1 TGC CCG i i i GAG 1 1 1 CTT 1 i l GCA 1 1 i GGA 1 1 1 TCG 1 1 1 i i i GGC I 1 1 CTC 1 1 i GAA 1 1 1 CGT 1 1 1 CCT 1 I 1 AGC CTC i i i CGC 1 1 t ATT 1 1 1 GGT CTT 1 1 1 GAC 1 1 1 i i i GAG 1 1 1 GCG I 1 1 TAA 1 í i CCA 1 1 1 GAA 1 1 1 CTG GAC i i i GGC t 1 1 AAT 1 1 1 TTC 1 1 1 GAT 1 1 1 GAT 1 I 1 i i i CTG 1 t 1 CCG 1 1 1 TTA 1 1 1 AAG 1 1 J CTA 1 i 1 CTA TGC i i i GAC J 1 1 GCA t 1 1 ATC 1 1 1 GTC 1 1 1 CGA 1 1 1 i 1 i ACG 1 1 I CTG 1 ( 1 CGT III TAG 1 1 I CAG 1 I 1 GCT CGT i i ( ACA ( í í CAA ( ( ( ATC 1 1 I GCC 1 I I CGC 1 í I ! i 1 GCA 1 1 1 TGT 1 1 i GTT 1 1 1 TAG 1 1 I CGG 1 1 1 GCG GAT i i i GGC 1 1 1 TGT i i i GTA 1 1 i GAA 1 1 1 GTA 1 l 1 i i i CTA 1 1 1 CCG i i 1 ACA 1 1 1 CAT 1 1 1 CTT t i 1 CAT CGC t i i CCC 1 t ! AGC t 1 1 ACT I 1 1 CGT 1 1 1 CCG 1 1 1 i i i GCG 1 1 1 GGG 1 1 1 TCG 1 1 1 TGA 1 1 1 GCA 1 1 1 GGC az előnyös plazmidok a következők: pIT!23, pITl25, pKC307, pIT208, pIT215, pIT217, plT219 és plT144; az előnyös transzformánsok a következők: E. coli K12 JA221/pIT123, E. coli K12 JA22t/pIT125, E. 8
