Dr. Murai Éva - Gubányi András szerk.: Parasitologia Hungarica 31. (Budapest, 1998)

RESULTS The soluble proteins of protoscoleces were resolved into six polypeptide fractions (Fl, F2, F3, F4, F5 & F6) by gel filtration (Fig. 1) having molecular weights (Mr) in the ranges of 90, 64.5,54, 34, 25 and < 17 KD respectively. Each protein fraction was dialysed and their diagnostic potential was determined with sera and immunocompetent cells taken at different days PI. Tube Number Fig. 1. Elution profile of the soluble proteins of protoscoleces from lung hydatid cysts on Sephadex G-200 column Fig. 2. In vitro percent macrophage migration inhibition at various phases of the prepatent period of E. granulosus infection Fig. 3. Dot blot analysis of the antigenic fractions (F1-F6) of protoscoleces and whole homogenate antigen (H) using sera collected at PI days 4, 8, 16,24,32 and 40; C: Control sera.