Dr. Murai Éva szerk.: Parasitologia Hungarica 23. (Budapest, 1990)

5. The quality of the formula developed by us was checked in an experiment in which LANIRAT-B baits, either free from sugar or containing 5 % sugar, mixed at Bábolna, were compared to an original LANIRAT bait manufactured by LIPHA. The results of this free-choice appetibility study (Table 4) show that the rats consumed almost as much of the sugar-free LANIRAT-B (47.3 %) as of the LIPHA LANIRAT (52.7 %, Fig. 3). Our LANIRAT-B containing 5 % sugar showed much higher acceptance, taking total bait consumption 100 %, the experimental animals consumed 68.9 % of LANIRAT-B and 31.1 % of LANIRAT marketed by CIBA-GEIGY. Mortality rate was 100 % in both free-choice experiments and the time of mortality ranged between 5 and 9 days. 6. The aim of the sixth series of experiments was to monitor changes in the rate and time of mortality in cases when the rodents were fed with the poisoned bait for a limited time instead of ad libitum feeding until death. In toxicity tests in which LANIRAT-B of 0.005 % bromadiolone content was fed for 96, 72, 48 or 24 hours both the rats and the mice showed 100 % mortality rate. When the rats were fed the poisoned bait for one day, they consumed an active ingredient dose of 3.1 mg/kg body mass on the average (Table 8). The lowest and highest active ingredient dose consumed was 1.9 and 3.3 mg/kg body mass, respectively. In mice the respective figures were much higher: when the poisoned bait was fed for one day only, the average bromadiolone uptake was 8.8 mg/kg body mass. The lowest and highest active ingredient uptake proved to be 3.3 and 11.7 mg/kg body mass, respectively (Table 5). MALE FEMALE LANIRAT-B I EPA Fig. 5: Sex-related differences in the consumption of LANIRAT-B bait