Dr. Murai Éva szerk.: Parasitologia Hungarica 16. (Budapest, 1983)

Using a Sephadex G-7 5 column, the estimated molecular weight of the inhibitor is about 6 000. (Fig. 1) ELUTION VOLUME (ml 150 100 C. pis.inh. P daubneyi inh. Cytochr. C J I I l—L ST I BSA j i I i I i i. l I

—^ 10 KP MOLECULAR WEIGHT Fig. 1: Estimation of molecular weight of the metabolic inhibitor of P. daubneyi on a Sephadex G-75 column. DISCUSSION The results of the experiments clearly indicate that P. daubneyi releases a trypsin-chymotrypsin inhibitor into the maintenance medium. The amount of inhibitor released during 6 h was well measurable, and it rose from 6 to 16 h, but not proportionally. The properties of this metabolic inhibitor of P. daubneyi are identical with those of the somatic inhibitor (JUHÁSZ, 1982). This fact suggest that the metabolic inhibitor is identical with the somatic one. Similar results were found for Ascaris suum (JUHÁSZ and NÉMETH, 1979) and the metacestode of Taenia pisiformis (NÉMETH and JUHÁSZ. 1981)., indicating that the inhibitors of the worms may function not only inside the body of the worms, but may be the source of metabolic inhibitors as well.