Dr. Murai Éva szerk.: Parasitologia Hungarica 14. (Budapest, 1982)
Routine flotation process was used for the detection of ova. From the surface of th3 flotation fluid centrifuged, 3 drops per sample were decanted by a ground glass rod to slides and examined under a light microscope of lumipan system in 3 fields of vision. The well-known ova of Aspiculuris tetraptera (1 to 4 per field of vision) could always be recognized, (4) Drugs . Mebenvef^ is a pharmaceutical product available as an anthelminthic in veterinary medicine . Its active agent is methyl5(benzoil)-l H-benzimidazol-2-yl-carbamate . Albendazole* contains methly-5(propylthio)-lH-benzimidazol-2-yl-carbamate as active agent. Mebenvet and albendazole are insoluble in water and hardly soluble in organic solvents, therefore, they were suspended with the aid of some drops ofTween-80 in isotonic NaCl solution when given through gastric tube. Mixing of both Mebenvet® (2%) or albendazole (4%) with the standard diet and its pelletation were done by LATI (Gödöllő, Hungary). Table 1. Coprological findings before and after treatment with Mebenvet (100 mg/kg body weight/day) for 2 days Mouse strains Number of animals in stock Number of animals examined Number of Aspiculuris tetraptera ova per field of vision x before after treatment Hriop: Swiss A/Sn/Hriop C57Bl/6/Hriop DBA /2/Hriop BALB/c/Hriop BDF1/Hriop 4 630 651 901 690 496 1 200 35 35 35 35 35 35 Total number of mice 8 568 Acute toxicity. Acute toxicity test have been carried out to find out if the special mouse strains are sensitive to Albendazole in a way similar to the cases reported in the literature . Two months old Hriop: Swiss outbred mice of both sex weighing 20-22 g were used, 5 per dose. Albendazole was administered through single gastric incubation or mixed with food given on two successive days. Mice were observed over 3 weeks after treatment. Treatment of the mouse stock. Prior to treatment the whole stock starved for 24 hours. During this time tap water was provided ad libitum. Then the mangers were filled with food containing drug to be replaced by normal food 48 hours later. In order to treat those mice which had been sucklings ad the time of the first treatment, feeding of the drug was repeated under identical conditions two months later. Measurement of food and drug consumption. Simultaneously with the treatment of the whole stock and also after 24 hours starving, 10 mice were given normal food and another group of 10 mice were fed with food containing 4% albendazole. On the basis of food consumption measured in a 48-hour period the amount of the drug ingested was calculated. Albendazole was first produced by SMITH KLINE Co., Philadelphia, U.S.A.
