Dr. Murai Éva szerk.: Parasitologia Hungarica 11. (Budapest, 1978)
origin, and killed simultaneously with the infected ones for experimental study. In every case, the entire gut was rinsed and extracted for enzyme assay, for tapeworm specimens can also occur in the distal gut segment, if the infection is massive. In vitro evidence of protease inactivation by living, intact tapeworms, and by inhibitors secreted by the latter has been presented earlier (PAPPAS and READ, 1973a, b; MATSKÁSI and JUHÁSZ, 1977). Bothriocephalus acheilognathi was not previously studied in this respect. In the present experiments the somatic extracts of B. acheilognathi inactivated the trypsin and chymotrypsin activities of fish gut rinsing fluids in vitro. This permits the conclusion that the tapeworms parasitic in the intestine of fishes depress the host's proteolytic enzymes also in vivo, above all through the production and secretion of specific inhibitors. References BARRINGTON, E.J.W.(1957): Alimentary canal and digestion. In: The Physiology of Fishes.Ed.: Brown, M. Academic Press. New York, p. 109-154. BAUER, O.N. - MUSSE LIUS, V. A. - NIKOLAEVA, V.M. - STRELKOV, Yu.A. (1977): Ihthyopatologiya. - Izdatelstvo pishchevaya promislennost. Moskva, pp. 431. BRAUN, F. - HELD, H. - RIEDMÜLLER, S. (1968): Vorläufige Ergebnisse vergleichendphysiologischer Untersuchungen an Coregonen verschiedener oberbayerischer Seen (Fettgehalt, Blutbild, Fermentspiegel, Parasitierung). - Archiv für Fischereiwiss. , 19. 114-130. HUMMEL, B.C.W. (1959): A modified spectrophotometric determination of chymotrypsin, trypsin and thrombin. - Canad. J. of Biochem. and Physiol. , 37. 1393-1399. MATSKÁSI, I. - JUHÁSZ, S. (1977): Ligula intestinalis (L. , 1758): Investigation of pierocercoids and adults for protease and protease inhibitor activities. - Parasit. Hung. , 10. 51-60. MATSKÁSI, I. - NÉMETH, I. (1977): Characterization of the proteolytic enzyme and enzyme inhibitors of Ligula intestinalis plerocercoids. - Summaries of the Third National Conference of Parasitology in Bulgaria, p. 153. MOLNÁR, K. (1977): On the synonyms of Bothriocephalus acheilognathi Yamaguti, 1934. Parasit. Hung., Hh_ 61-62. ONISHI, T. - MUR AY AMA, S. - TAKEUCHI, M. (1973a): Sequence of digestive enzyme levels in carp after feeding-I. Amylase and protease of intestinal content, hepatopancreas and gall-bladder. - Bull, of Tokai Regional Fisheries Res. Lab. , 75. 23-31. ONISHI, T. - MURAYAMA, S. - TAKEUCHI, M. (1973b): Sequence of digestive enzyme levels in carp after feeding - II. Protease in activated and zymogen forms of intestine, hepatopancreas, gall-bladder and spleen. - Bull, of Tokai Regional Fisheries Res. Lab. , 75_. 33-38. PAPPAS, P. W, - READ, CP. (1972a): Trypsin inactivation by intact Hymenolepis diminuta. J. of Par. , 58^ 864-871. PAPPAS, P.W. - READ, CP. (1972b): Inactivation of OC-and ß-chymotrypsin by intact Hymenolepis diminuta (Cestoda). - Biol. Bull., 143. 605-616. PHILLIPS, A.M. (1969): Nutrition, digestion and energy utilization. In: Fish Physiology. Ed.: Hoar, W.S. - Randall, D.J. Academic Press. New Yc ondon, pp. 402. RÁGY ANSZKI, M. - JON ÁS, E. (1977): A fehér busa (Hypopht 1 ays molitrix.ValJ proteolitikus emésztő enzimének vizsgálata. - II. Halászati ányosNapok, Szarvas, 42-51.
