Dr. Kassai Tibor - Dr. Murai Éva szerk.: Parasitologia Hungarica 10. (Budapest, 1977)
The reliability of the applied technique was checked by calculation of the variation coefficient for 20 replica measurements on identical materials. The coefficients obtained for chymotrypsin activity, chymotrypsin inhibitor activity and trypsin inhibitor activity were 24, 21 and 24%, respectively. Fig, 1 U/g 15 Chymotrypsin-like enzyme and chymotrypsin inhibitor activity of plerocercoids 10 —x—x —x —x —x— -x —x —v — xKRT 24 KRT 48 worms kept in culture media TS 24 worm homogenates mammalian chymotrypsin activity chymotryptic activity of worms Mammalian enzyme activity after the reaction of parasite inhibitor at 37°C: x — x —-x — x- measured prompt, — o —o —o— measured after 15 minutes incubation — •— measured after reaction of parasite inhibitor (worm extract) treated for 30 minutes at 56°C. Results Proteolytic activity of the parasites Chymotrypsin-like BTEE-degrading proteolytic activity was demonstrable in the nutrient medium of Ligula plerocercoids and extracts of larval and adult stages as well. Activity assays in the different media have shown lowest activity in the Tyrode solution noted for low buffer 5 3
