Dr. Kassai Tibor - Dr. Murai Éva szerk.: Parasitologia Hungarica 9. (Budapest, 1976)

Examination of Paramphistomid Species of Bulgarian Domestic Ruminants Dr. Ottó SEY — Dr. Yurij VISHNYAKOV Department of Zoology, College of Education, Pécs, Hungary — Department of Parasitology, University of Veterinary Science, Sofia, Bulgaria "Examination of paramphistomid species of Bulgarian domestic ruminants. " Sey, O. - Vishnyakov, Yu. - Parasit. Hung. 9^ 25-29. 1976. ABSTRACT. Paramphistomid specimens collected in nineteen localities of Bulgaria from cattle, sheep and buffalo proved to belong to a single species: Paramphistomum daubneyi Dinnik, 1962. Characters to be taken into account when differentiating P. daubneyi from closely related species are discussed in detail. Material and Methods In 1974/75 an extensive collecting was carried out in Bulgaria in order to survey the species of Paramphistomum in domestic ruminants. The test material was collected from cattle in Burgas, Chepelare, Godech, Ihtiman, Kavarna, Kirnalovo, Kotel, Mihailovgrad, Momchilgrad, Rousse, Sliven, Topola, Topolovgrad, Torgovishche, Vidin; from sheep in Kavrikirovo, Kirdzhali, Kremene, Oborishte, Vidin and from buffalo in Sliven. Flukes after having been removed from the rumen were fixed and stored in 70% alcohol, then median sagittal sections were prepared with the usual histological method. Of every sample five specimens were sectioned and another five were dissected to detect the position of the ends of caeca and convolutions of the pars musculosa (number of specimens were limited). Although several authors have examined Paramphistomids of Bulgaria (VISHNYAKOV and IVANOV, 1964; VISHNYAKOV et al. 1971; POPOV et al. 1967; MIHAILOVA et al. 1974; KOTRLA et al. 1974) their conception concerning the established species is by no means uniform. Result and Discussion The identification of the present material was based on gross and histomorphology, at the same time specificity to intermediate hosts was also taken into consideration. Samples of the available Paramphistomid material proved to be a single species: Paramphistomum daubneyi Dinnik, 1962 all but one deriving from cattle in Kavarna, to be discussed later. In the Bulgarian literature VISHNYAKOV et al. (1971) were the first to report the occurrence of a P. daubneyi - like species from sheep. P. daubneyi

, belonging to the group of species having Microbothrium type of genital atrium shows the closest similarity to P. microbothrium Fischoeder, 1901 and P. clavula Näsmark, 1937 reported in Bulgaria (MEREMINSKIÍ and VISHNYAKOV, 1969; VISHNYAKOV et al. 1971; MIHAILOVA et al. 1974). Owing to the similarity of P. daubneyi