Dr. Kassai Tibor - Dr. Murai Éva szerk.: Parasitologia Hungarica 8. (Budapest, 1975)

Investigations on the Efficacy of Some Anticestode Compounds against Echinococcus granulosus in the Dog * Dr. Tibor KASSAI — Dr. Péter REDL — Csilla TAKÁTS — Dr. Ferenc HOLLÓ — Dr. Károly MAGYAR — Dr. János GYENES Helminthological Reserch Laboratory, Department of Parasitology, Veterinary University, Budapest — PHYLAXIA Veterinary Biologicals and Feedstuffs Ltd., Budapest "Investigations on the efficacy of some anticestode compounds against Echinococcus granulosus in the dog" - Kassai, T. - Redl, P. - Takáts, Cs, - Holló, F. - Magyar, K. - Gyenes, J. - Parasit. Hung. 8. 29-35. 1975. ABSTRACT. The efficiency of single treatments with bunamidine hydroxynaphthoate (BHN, Burroughs Wellcome and Co. ), bunamidine hydrochloride (BHC1, "Scolaban" Burroughs Wellcome and Co. ), niclosamide (NA, "Dayermin" Chinoin) and cantrodifene (CF, "Lopatol", CIBA-GEIGY) against 6-week old E. ganulosus was tested on 113 experimentally infected dogs'. BHN at 100 mg/kg removed 95.1-97.2 per cent of the worms from 89 per cent of the treated dogs. FoUowing treatment with NA or CF, considerable worm reduction was recorded in 28 and 14 per cent of the animals, respectively, while results of the BHCl-treatment were inconclusive. BHN in biscuit formulation was found to fulfill practical requirements of control schemes involving regular dosing. Despite many previous research endavours to acquire an appropriate chemotherapeutic agent against Echinococcus granulosus, - treatment of canine echinococcosis remains a problem deserving of attention, for even those few compounds that have been recommended against this parasite have been indicted for inadequate efficiency or undesirable side effects. Since 1965 data have accumulated on the anti Echinococcus activity of several naphthalene derivatives, of which bunamidine (N : N-di-n - butyl-4 -hexyloxy1 - naphthamidine) hydrochlor.ide

x has become widely known on account of its reliable efficiency (BALTZLY etal.

, 1965; FORBES, *966; ZUKOAflC and WIKERHAUSER, 1967; GEMMEL and SHEARER, 1968; BLOOD et al., 1968; DEMIDOV et al., 1974; AZIMOV és AMINZHANOV, 1974). However, laboratory and field studies, conducted in New Zealand, revealed toxic, sometimes fatal complications after treatment with bunamidine hydrochloride (THOMSON, 1973; MENRATH et al.

, 1973). In laboratory trials a water-insoluble salt of bunamidine, bunamidine hydroxynaphthoate, also proved very efficient, and less toxic even at higher and repeated doses (SHEARER and GEMMEL, 1969; NIKI TIN et al., 1973; GEMMEL and OUDEMANS, 1974/a). Niclosamide (N-/-2-chlor-4-nitrophenyl/-5-chlor-salicyl-amide) is known to be highly effective against common tapeworms of the dog. However, conflicting results have been reported about its action against E. granulosus (KURELEC and RIJAVEC, 196.1; FORBES, 1963; DELAKetal.

, 1963; BABOS et al.

, 1964). Recently a broad spectrum anthelmintic, cantrodifene (4-isothiocyano-4*-nitro-diphenyl ether)

xx has also been claime.d to be highly effective against E. granulosus in the dog (BORAY, von ORELLI and SARAS IN, 1974; GEMMEL and OUDEMANS, 1974/b). * Presented at the Second European Multicolloquy of Parasitology, 1-6 September, 1975, Trogir, Yugoslavia. X "Scolaban", Burroughs Wellcome and Co. XX

"Lopatol", CIBA-GEIGY