Matskási István (szerk.): A Magyar Természettudományi Múzeum évkönyve 94. (Budapest 2002)

Czuzdi, Cs. ; Szlávecz, K.: Diplocardia patuxentis sp. n., a new earthworm species from Maryland, North America (Oligochaeta: Acanthodrilidae)

MATERIALS AND METHODS Sampling primarily took place in mixed deciduous forests, forest edges and riparian zones. The most common trees in this area include several species of oak (Quercus nigra, Q. velutina, Q. alba, Q. rubra), tulip poplar (Liliodendron tulipifera), red maple (Acer rubrum), and sweetgum (Liquidambar styraciflua) Earthworms were collected by formalin extraction, digging and handsorting. Animals were killed in 75% ethanol, fixed in 4% formalin for several days and preserved in 75% ethanol. Parallel collections of specimens are deposited in the earthworm collection of the Johns Hopkins University, Baltimore, MD, USA and in the soil zoology collection of the Hungarian Natural History Museum, Budapest, Hungary. TAXONOMY Diplocardia patuxentis sp. n. (Figs 1-4) Diagnosis - Length 40-63 mm, diameter 2.5-3.5 mm. Pigmentation lacking. Prostomium epilobous, first dorsal pore in the intersegmental furrow 10/11. Clitellum annular, on segments 13-18. Female pores in 14 presetal. Prostate pores on 19, 21 in setal line b. Male pores on 20, in seminal grooves. Penial setae and copulatory setae present. Unpaired pad-like genital markings in 8, 9, 11, 22, 23, between setae b. Spermathecal pores in intersegmental furrow 7/8, 8/9. Muscular gizzards united in 5-6. Testes paired in 10, 11 enclosed in perioesophageale testis sac. Seminal vesicles in 9, 12. Last hearts in 12. Dorsal blood vessel single throughout. Oesophagus widened in 15-16, with low but dense longitudinal folds. Intestinal origin at 17/18. Description - Holotype 55 mm in length and 3 mm in diameter, segments 110. Paratypes 40-63 mm long and 2.5-3.5 mm wide. Body cylindrical throughout, 71-113 segments. Setae paired, setal formula at segment 25; aa:ab:bc:cd:dd=3.3:

1:2:1:13.3. Prostomium epilobous, 1/2 open. Segments 11,12 triannulate, after the clitellum slightly bi- or triannulate. Color pale, pigmentation lacking. First dorsal pore in 9/10 closed, in 10/11 open. Nephridial pores on the leading edges of segments in the setal line d. Spermathecal pores minutes, intersegmental in 7/8, 8/9 open in setal line a. Female pores paired presetal on 14, somewhat median to setae a. Clitellum 13-18, annular. Prostate pores at the end of seminal grooves on 19,21 in setal line b (Fig. 1). Male pores on 20. Unpaired pad-like genital markings between setae b on 8, 9, 11, 22, 23. Setae a-b on 8 and 9 frequently missing, if present modified. Copulatory setae small, straight, about 0.2 mm in length with several scale-like ornamentation (Fig. 2). First septum visible 5/6. Septa 6/7, 7/8, 8/9 slightly muscular. Two oesophageal gizzards fused in 5-6. No obvious calciferous glands, but the oesophagus widened in segments 15-16 with densely arranged, low to middle high longitudinal folds. Intestine begins in segment 18. Thyphlosole about one third in diameter of the intestine, gradually decreasing and ends at the segment 47. Dorsal blood