Matskási István (szerk.): A Magyar Természettudományi Múzeum évkönyve 93. (Budapest 2001)
Csapó, J., Bernert, Zs. , Csapó, Zs. , Pohn, G. , Csapó-Kiss, Zs. , Költő, L. , Szikossy, I. ; Némethy, S.: Introduction of amino acid racemisation based age estimation into paleoanthropological [sic] research
(1987). We used a Kromasil octil (C8) loaded analytical column of 25x4.6 mm internal diameter and 5 mm particle size. To extend the lifespan of this column we inserted a safety column (RP8, Newguard, internal diameter 36x4.5 mm, particle size 7 mm, Brownlee) between the sample feeder unit and the analytical column, and we built in a cleansing column (CI 8, internal diameter 36x4.5 mm, 20 mm particle size Rsil) between the pump and the sample feeder. To separate enantiomers we utilised a two component gradient system with the following components: B: acetonitril in an A:40% methanol phosphate puffer (9.5 mM, pH:7.05). The speed of the flow was 1 cmVminute and gradient changed as a function of time like this: Time (minute) 0 10 35 55 56 74 75 A% 95 95 83 72 67 67 U2 B% 5 5 17 2S 33 33 3 S Detection - Maximum stimulus was 342 nm and maximal emission was 410 nm in the fluorescent detection of OPA/TATG derivatives. Storage and evaluation of data - These were carried out utilising a LaChrom D-7000 System Manager device that made the presence of D-amino acids beside L ones measurable and évaluable even in extremely small concentration. We evaluated the data produced by linear regression. D/L ratios and ln(l+D/L)/(l-D/L) of aspartic glutamic acids were plotted as a function of time by drawing so called calibrating diagrams. RESULTS OF AGE ESTIMATION BASED ON THE D-ASPARTIC , AND D-GLUTAMIC ACID CONTENTS OF TEETH Our research project was carried out in collaboration with the Institute of Odontology and Earth Sciences Centre of Göteborg University. We analysed two recent tooth sample series to establish the so called calibrating diagrams. In 1998-1999 we gathered 22 teeth from the dental surgery of Pannon Agricultural University's Faculty of Animal Sciences in Kaposvár and we measured the D- and L-aspartic acid contents of them. When planning the sample we attempted to include individuals with in the largest possible age envelope (17-62 years), and we also tried to select enough individuals from each age group to have a comprehensively representative sample. Table 4 contains the data produced by analysing the Kaposvár dental sample of 1998. We calculated ln(l+D/L)(l-D/L) correlations both for aspartic and
