Matskási István (szerk.): A Magyar Természettudományi Múzeum évkönyve 88. (Budapest 1996)

Szedlay, Gy., Jakucs, E. , Bóka, K. ; Boldizsár, I.: Macro- and micromorphological characteristics of Ganoderma lucidum Karsten strains isolated in Hungary

YOUNG (1973) these are 9-13 x 6-8 (im, as to ADASKAVEG & GILBERTSON (1988) 10.0-11.8 x 6.8-7.9 rim, and as to WANG & HUA (1991) 7.0-12.0 x 6.0-8.0 um. The hyphal system is trimitic. Isolates of Ganoderma lucidum regularly form chlamydospores in mycelial culture and have an average growth rate of 7.8 mm/day at its optimum temperature range of 3034 °C (ADASKAVEG & GILBERTSON 1986). The aim of our work was to investigate the morphological and cultural characteristics of Ganoderma lucidum strains isolated from different habitats of Hungary and to compare them to isolates originating in other countries. The taxonomic value of characteristics regularly used to make distinctions between the species and between strains of the species itself were examined. MATERIALS AND METHODS Isolates Basidiocarps of Ganoderma lucidum were collected from various regions of Hungary. Collection informations (host relationships, collection localities) are presented in Table 1. Mycelial cultures were obtained by cutting out small pieces from the inner layers of basidiocarps under steril conditions and were maintained at 26 °C on malt extract agar (MEA) or on potato dextrose agar (PDA). The strains were deposited in the Plant Collection of Hungarian Natural History Museum and in the Department of Plant Anatomy of Eötvös Loránd University. Light microscopy Préparâtes for light microscopy were made from the fresh mycelium grown on the agar slides or spores taken from the fruitbody. One sample from both the margin (young part) and the middle (old part) of two- and six-weekold cultures grown on MEA and PDA was mounted in lactophenol (lactic acid:glycerol:phenol:distilled water, 2:4:2:2), covered with cover slips and sealed. In some cases staining with 1% anilin-blue was applied. The préparâtes were examined by Nomarski interference contrast microscope (Olympus). Photos were taken using an immersion objective of lOOx magnitude. Scanning electron microscopy (SEM) Mycelium, basidiospores and pilocystidia were examined by Hitachi-2360-N scanning electron microscope. For SEM investigations mycelium on agar cubes were fixed in 2.5% glutaraldehyde, postfixed in 1% osmium tetroxide, washed in 0.07 M phosphate buffer (pH 7.2), dehydrated stepwise in ethanol (25%, 50%, 75%, 90%, 96%, abs.) and amylacetate. The samples were critical point dried and coated with carbon and gold layers. Spores were air dried from xylene before coating. Spore Table 1. Sources of Ganoderma lucidum strains Isolate Locality C17 Budakeszi C64 Budakeszi CI 14 Mátra Mts CI 15 Pilis Mts CI 16 Pilis Mts CI 18 Kópháza-Nagycenk CI 19 Kópháza-Nagycenk CI 20 Budakeszi C122 Buda Mts: Normafa CI 23 Mátra Mts C124 Mátra Mts C125 Mátra Mts C126 Mátra Mts C127 Buda Mts: Farkas-völgy C129 Buda Mts