Kaszab Zoltán (szerk.): A Magyar Természettudományi Múzeum évkönyve 66. (Budapest 1974)

P. Komáromy, Zs.: Studies on the aerophytic Chorhormidium flaccidum (Kütz.) Fott (Ulotrichales)

ments with sterile injection syringe; the needle was sterilized after each injection. The cultures were evaluated both, macro- and microscopically after the 3rd, 7th and 14th day. Series III was inoculated on the 10th of October, macroscopic evaluation again took place on the 3rd, 7th and 14th day. It was impossible to microscopically evaluate the large number of samples in one day, thus, in subsequent days the measurements were made in pairs. Series IV was inoculated on the 5th of November. However, not every culture had its corresponding parallel, but only to so-called "pure lines" were further inoculated, thus, series IV comprised eight cultures. The microscopic examinations were done in pairs on the subsequent days. The changes of cell dimensions of the alga film and coat were recorded in the first 10 days and later plotted (Plate I, Figs. 4—5). Examination of cultures inoculated on Detmeragar — It was assumed that the two varieties (film and alga coat) segregate sharply from each other only in the fluid cultures, thus, the examinations had to be made primarily on liquid cultures. However, Chlorhormidium flaccidum (Kütz). Fott comes from an aerophytic environment, thus, the medium might be more acceptable than a culture of liquid medium. In order to observe thallus formation, from series I and II inoculation was made on 1.5% Detmer-agar and Detmer-agar containing 1% glucose. Results Examination of liquid cultures — In the course of subsequent inoculations, it was not possible to isolate the film layer and alga coat formed at the bottom of the culture vessel. After inoculation both regenerates the missing filament type in a matter of a few days. Supposing that both the thin layer and the mass of filaments collected at the bottom of the fluid "include" physiologically different filaments, and after inoculation these produce the other — undesired — culture, it was endeavoured to separate the two types of filament by a vigorous shaking of the culture vessel, by which it was expected that they would disperse. One series of the parallel inoculations produced from series III was shaken (b), the other was kept as a control (a). On the 3rd, 7th and 14th day after inoculation the presence ( + ) or absence (—) of colonies was macroscopically established. The production of cultures compared to one another was numbered from 1 to 3. Examining the production of the individual cultures certain differences were found, which could only be traced back to the different quantities of the inoculation. The experiments verified the assumption. After inoculation in the shaken cultures (group b), the earlier established filament connections broke up, and the individual types of filaments separated well after the 3rd or 4th day. Microscopic measurements this time showed a small scattering of values. On the other hand, the new cultures in group a kept up their original tendency. The development of the alga coat and the superficial layer took place 7—14 days after inoculation. The macroscopic observations suggested that at the development of the film layer and the alga coat, the physiologically diverging filaments spacially segregate one from the other. This is further proved by the microscopic examinations, too. Differences occurred in the arrangement of the filaments, in the length and vacuolization of the cells forming a filament. In the superficial layer long, parallel running filaments adhere closely to each