Kaszab Zoltán (szerk.): A Magyar Természettudományi Múzeum évkönyve 65. (Budapest 1973)

Kováts, D.: Anatomical investigations on the vegetative system of Lithospermum purpureo-coeruleum L.

ANNALES HISTORICO-NATURALES MUSEI NATION ALIS HUNGARICI Tomus 05. Budapest 1973. Anatomical Investigations on the Vegetative System of Lithospermum purpureo-coeruleum L. By D. KOVÁTS, Budapest Abstract — Description on the basis of the author's investigations and a critical analysis of literature of the epigeous and hypogeous shoots, foliage leaves and anatomical characteristics of Lithospermum purpureo-coeruleum L. The general anatomical features of Lithospermum purpureo-coeruleum L. are already known (SOLEREDER 1899, JODIN 1903, METCALFE & CHALK 1965). These works discuss the charac terization of the Boraginaceae and only rarely mention the genus Lithospermum, while actual studies concerning the species are still more seldom. Papers published so far have treated the seedling and the development of shoots of root origin of Lithospermum purpureo-coeruleum L. (KOVÁTS 1971, KOVÁTS-STIEBER 1972). The present paper deals with the anatomical characterization of the developed hypogeous and epigeous shoots and foliage leaves, as well as the critical review of the respective literature. Material and method The study material was contiguously collected since 1967, mostly in the Mts. Buda near Budapest (Csiki-hegyek, Remetehegy), and in the Transdanubien and northermchairs of the Central Range (Mts. Vértes, Pilis, and Börzsöny). The parts to be cut were fixed in NAVASIN'S fixing mixture. Most of the material was prepared by hand-made slides, under a Zeiss SM XX Cytopplast microscope, the slides made by razor blades. The slides were then treated with Javel lye (,,Hypo") solving the plasm, and the strong lye neutralised by acetic acid. In both microforme and hand-made slides vesuvinmalachite green double staining or toluidine stainings were a/pplied. The slides were made permanent in watery glycerine and framed with lacquer. Skin preparations from leaves and shoot epiderms were also made. Two methods were applied ; without describing them, only literature references are here made (ÚJHELYI 1954a; SÁRKÁNY & SZÁLAI 1964, KVACEK 1966). Examinations were made under a Zeiss Nf PK microscope ; microphotographs were also made by Zeiss automatic microphotographing equipment, planachromate objectives and MF compensation projectives, on 15 din ORWÖ roll films. Anatomical characteristics of the hypogeous and epigeous shoot axes (stem) Epiderm Cross-sections of epidermal cells of epigeous shoots largely circular, often slightly elongated tangentially (Plate I, Figs. 3, 4; Plate II, Fig. 1). In longitudinal sections, epidermal cells elongated in longitudinal direction of shoot (Plate I, Fig. 1). Stomata mostly without accessory cells, therefore of Ranunculaceae type. Stomata with accessory cells also occurring, these latter situated parallel with stoma (Rubiaceae type). Unicellular cystolith hairs and hairless cystoliths also Ann. Hist.-nat. Mus. Nat. Hung., 1973, 65