Kaszab Zoltán (szerk.): A Magyar Természettudományi Múzeum évkönyve 64. (Budapest 1972)

Babos, M.: Investigations on the spore germination of Coprinus radians Desm.

Ozonial hyphae (under microscope) brownish yellow, consisting of smooth, thin-walled cells. Herbarial data from the myeologieal collection of the Botanical Department, Hunga­rian Natural History Museum: Sajószentpéteri, Com. Borsod-Abaúj-Zemplén, April, 1930, leg. TiEES ; Szekszárd, Com. Tolna, on rotting rafter, 11 Dec, 1929, leg. L. HOLLÓS; Szenta, Com. Somogy, in oakwood, 10 Oct., 1967, leg. M. BABOS — G. BOHUS; Pusztavacs, Com. Pest, in locust-tree wood, on rotting Bobinia branch, 30 April, 1967, leg. M. BABOS — E. VÉSSEY; Csévharaszt, Com. Pest, in locust-tree wood, on rotting Robinia branch, 20 June 1967, leg. M. BABOS — G. BOHUS—I. KONECSNI — E. VÉSSEY; in locust-tree wood, on decaying Populus branch, 21 July, 1970, leg. M. BABOS -I.RIMÓCZI - E. VÉSSEY; Budapest : Pestlőrinc, on decaying Acer branch, 14 May, 1967, leg. M. BABOS — E. VÉSSEY ; Budapest: Újpest, on decaying wood substance, 23 June, 1969, leg. L. NAGY; Budapest: Megyer, on decaying wood substance, July, 1969, leg. L. NAGA t ; Budapest: Makkosmária, in hornbeam-oakwood, on decaying Quercus branch, 20 Oct., 1968, leg. M. BABOS — E. VÉSSEY; Budapest, in a garden, on decaying branch fragments of Juglans regia, 20 April, 1967, leg. G. BOHUS. Walnut branches, collected on 20 April, 1967, 5-6 cm in diameter and coated with ozonium, served for the experiments. The bits of wood were soaked and kept in glass cylinders rich in humidity (95%) ; fruitbodies of the fungus developed regu­larly in laboratory conditions, to wit: April —May, 1967 (numerous specimens); 13 July, 1967 (2 specimens) ; Sept., 1967 (many) ; Oct., 1967 (3 specimens) ; 11 May, 1968 (3 specimens); 14 May, 1968 (3 specimens); 18 March, 1969 (2 specimens); 26 Oct., 1970(1 specimen); 18 Dec, 1970 (2 specimens) ; 14 Jan., 1971 (1 specimen). The rate of spore germination was observed primarily on malt-agar culture media (3% malt extract+2% agar-agar). Spore smears were made on the culture substrate placed in the hollow of a slide ; the study of temperature effects (at temper­atures 24, 20.5-21, 14-15, 8.5-9 and 3-4°C) on the release of spore germination was made in the same w r ay. In studying the stimulating effect of the culture media, the following substan­ces were drawn into the experiments : 3% malt extract +2% agar-agar distilled water+malt extract in various quantities (30/00, 6%„, 1.5%, 3%) (pH 5.4) distilled water+ 1.5% malt extract + n/ 10 NaOH (pH 7.7 and 7) distilled water-fn/10 NaOH (pH 7.8 and 7) distilled water Investigating the role of the pH values, the culture media were set, bv dosing n/10HCl and n/10 NaOH, at pH 2.9, 3.9, 5.1, 6.0. 7.0, and 7.7; distilled water served as control. The drought tolerance of the ozonium could be established after dry-storing the interwoven w r ood material for 6 and 16 months. In favourable humidity conditi­ons, fruitbody development commenced quicker or slower, according to the dura­tion of the dry period. 6 Természettudományi Múzeum Évkönyve 1972.

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