L. Forró - É. Murai szerk.: Miscellanea Zoologica Hungarica 5. 1989 (Budapest, 1989)
Sey, O.: Laboratory model for studying experimental fascoilopsiosis
excretory bladder and ducts, filled with granules, are easily seen. The life-span of metacercariae was 70 days at room temperature and in moisture (NGUYEN THI LEE and SEY, 1985) but their infectivity considerably decreased after 45 days. Development in definitive hosts Our examinations embraced three pigs and ten rabbits as definitive hosts. They were fed on the metacercariae in gelatine capsule with the appropriate number of metacercariae (pigs - 500, rabbits - 200 each). All experimental animals became infected. Rabbits were sacrificed in every two months. It was observed that the fluke did not survive the infection in rabbits after ninety five days. In this age flukes were 15-17 mm in length having no eggs. Accordingly, rabbits did not seem to be model animals in a study of experimental fasciolopsiosis. WU (1937) and recently MALVIYA (1985), however, demonstrated, that F. buski can reach its maturity in rabbit, as some of these flukes contained eggs, but they did not test whether these eggs were really fertile or not. Our observations on the development of F. buski in pig were similar to those presented by BARLOW (1925), NAKAGAWA (1921), NÄKAGAWA and SUZUKI (1923), YOUNG (1936), TRIPATHI et al. (19731 and NGUYEN THI LE and SEY (1985). The first eggs were discharged in 50- 65 days after infection, but the greatest number of eggs (3 000-4 500 EPG) was layed in 90- 120 days old infection and as time passed, the daily egg-production considerably decreased. When the last donor was sacrificed 370 days after infection, the take was 15 per cent and a few number of eggs were found in the uterus. REFERENCES BARLOW, C.H. (1925): The life cycle of human intestinal fluke Fasciolopsis buski (Lankester, 1856). - Amer. J. Hyg. Monogr. Ser., 4: 1-98. MALVIYA, H. C. (1985): The susceptibility of mammals to Fasciolopsis buski. - J. Helminthol., 59: 19-22. NAKAGAWA, K. (1921): On the life cycle of Fasciolopsis buski, Lankester. - Kit. Arch.Exper. Med., 4: 160- 167. NAKAGAWA, K. and SUZUKI, S. (1923) : Investigation of the life-history of Fasciolopsis buski (second report). - J. Med. Assoc. Formosa, 22: 228-232. NGUYEN THI LE and SEY, O. (1985): Infectivity of metacercariae of Fasciolopsis buski (Lankester, 1857) and their development in definitive host. - Parasit, hung., 18: 11-15. TRIPATHI, J.C., SRIVASTAVA, H. D. and DUTT, S. C. (1973) : A note on experimental infection of Helicorbis coenosus and pig with Fasciolopsis buski. - Indian J. Anim. Sei., 43: 647-649. YOUNG, S. (1936): Studies on the final host of Fasciolopsis buski and its development in the intestine of the pig. - J. Shanghai Sei. Inst., 4: 225-236. WU , K. (1937): Susceptibility of various mammals to experimental infection with Fasciolopsis buski (Trematoda: Fasciolidae) . - Ann. Trop. Med. Parasit., 31: 361-369. Author's address: Dr. Ottó SEY Department of Zoology University of Agricultural Sciences, Keszthely, Deák Ferenc u. 16. H-8361 Keszthely HUNGARY
