Dr. Éva Murai szerk.: Miscellanea Zoologica Hungarica 3. 1985 (Budapest, 1985)

Sulgostowska, T.; Giziewicz, J.: Influence of material extracted from Diploposthe laevis (Bloch) and D. bifaria (Siebold in Creplin) (Cestoda) on tapeworm Hymenolepis diminuta (Rudolphi) in rats

whereas it was insoluble in water. Heating of the oil with water up to 40°C, with intense stirring, yielded an emulsion stable for several hours. The emulsion was extracted four times with 100 ml of chloroform. The combined chloroform extract was liberated from chloroform by evaporation of the oil which was dried in a dessicator under vacuum. This procedure provided a yield of oil of about 10% against the dry weight of tapeworms. For comparison, the material comprising random species of tapeworms collected from duck Anas genus was subjected to an identical treatment. Preparation of post-acetone water extract (WE) from the tapeworms U. laevis and D. bifaria Tapeworms previously extracted with acetone were homogenized with a 10-fold volume of water in a glass homogenizer. The solid parts were removed by centrifugation

, and volumes of the supernatant corresponding to 500 mg dry weight of the tapeworms were taken for the biological activity tests. WE was also prepared from random tapeworm species. Biological tests Hundred Wistar rats (1 year old) were experimentally infected with 4-5 Hymenolepis diminuta (Rud., 1819) larvae per rat. Four weeks after the experimental infection, rat faeces were examined for the presence of H. diminuta. In the biological tests the aqueous emulsion of AEO and WE from D. laevis and D. bi faria as well as from the random tapeworms, were administered intragastrically to the rats with a probe. Table 1, Effectivity of tapeworm extracts on Hymenolepis diminuta population in rat Experimental groups 30 rats treated with AE0

1 30 rats treated with WE 15 rats treated with AE0

2 15 rats treated with WE

2 10 rats control Results H. diminuta ejected, rejected, compactly curled no effect slightly effected no effect H. diminuta uneffected Effective % 100 % 0 % 20 % 0 % AFOj = Acetone extracted oil from Diploposthe laevis and D. bifaria WEj = Water " " " " " AEO2 = Acetone extracted oil from mixed tapeworms of duck WE

2 = Water " " " " " " " After definite periods the rats were sacrificed and dissected in order to determine the reaction of tapeworm H. diminuta to the investigated materials. The behaviour of H. diminuta tapeworms in infected rats (10 specimens) which were not treated with the investigated materials served as control. RESULTS In the first experiments the dose of the AEO, emulsion required to exert a toxic action on H_. dim

inuta was determined. The applied doses of AEO^ corresponded to 125, 250, 500 and 800 mg of dry weight of D. laevis and D. bifaria. It was found that a perceptible toxic effect was exerted by a dose of AEO^ corresponding to 500 mg dry weight of tapeworms; this amount could be obtained from 4-7 tapeworms (Table 1). The toxic effect on H. diminuta manifested itself in the following five ways: 1) translocation of tapeworm from the anterior of the jejunum to its further parts, and even to the