Marisia - Maros Megyei Múzeum Évkönyve 36/1. (2016)
Botany
In vitro assay of antibacterial activity of three medicinal plants Fig. 4: Chromatographic fingerprint viewed at UV 366 nm. The sequencing of standard plates and extract compounds: 1- ferulic acid, 2- gallic acid, 3- chlorogenic acid, 4- quercetin, 5- hydroethanolic extract of E. caryophyllata, 6- extract of A. absinthium-, 7. extract of J.nigra, 8-rutin, 9- kaempferol. Absorbance layer appears in dark color and spots fluorescente. Following the analysis of the chromatographic fingerprints in hydro ethanolextract of Я caryophyllatavr&re identified four synthetic compounds: ferulic acid, gallic acid, quercetin and kaempferol. In the extract of J. nigra were identified three compounds: gallic acid, quercetin and kaempferol and in A. absinthium extract, three compounds: gallic acid, chlorogenic acid and quercetin. Quercetin flavonoid and gallic acid phenol were present in all the extracts studied, kaempferol flavonol was identified in the extract of E. aryophyllata and J. nigra extract and ferulic acid phenol was identified only E. caryophyllata extract. Chlorogenic acid was specific only to A. absinthium extract. From the chromatographic analysis that can observe that it using either the visible light or UV detection at 254 nm is not sufficient to extract the fingerprints. Only a few compounds can be detected in visible light and 254 nm UV. Almost all of the compounds absorbing at 254 nm can also be detected in visible light. More than this, the fingerprints obtained in visible light and at UV 254 nm, just as well, do not allow a good differentiation of evidence. The most complete information about the constituent compounds of the extracts was obtained by detection at UV 336 nm in fluorescence. The qualitative analysis by TLC is relatively simple, being a technique used in many laboratories for the separation of various mixtures of organic compounds. Another advantage of this technique would be that enables simultaneous separation of a large number of samples (samples 50—60). Quantitative evaluation of the antibacterial activity of hydroethanolic extracts of E. caryophyllata The antimicrobial activity of plant extracts is the first demonstrated pharmacological property for these. The medicinal plants have a great potential to produce new drugs of great benefit to mankind. Higher plants are capable of synthesizing unlimited numbers of highly complex and unusual chemical substances [Farnsworth, 1988]. The qualitative evaluation of antibacterial activity of plant extracts on solid media is an estimate because the extent of absorption of the compound by the medium is unknown. The calculation of MIC via dilution method in 96-well plates with the liquid media yielded values between 7.8 and 500 pig/ mL for 47
