Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)

Botany

ANTIBACTERIAL ACTIVITY OF COMPOUDS DERIVED FROM HIDROETHANOLIC EXTRACTS OF JUGLANS NIGRA

Luminija ROMAN1, Anamaria HOSU2, Cristiana VASILIU3, Horajiu ROMAN4, Gheorghe CIUCÄ5, Grigore MIHÄESCU1

' Faculty of Biology, University of Bucharest, Splaiul Independenfei 91—95 Sector 5, RO-76201 Bucharest;

2 Faculty of Chemistry and Chemical Engineering ,,Babe$-Bolyai” University of Cluj Napoca,Strada Arany János

11, R0^i00028 Cluj Napoca;

3 Social worker, London, Flither Green, 13 SE, 54D, Lewisham;

4 Faculty of Geology and Geophysics, University of Bucharest, Strada Traian Vuia 6 Sector 2;

5 Faculty of Electrical Engineering Bucharest, Tärgu Jiu, Calea Eroilor, RO-210163 Tárgu Jiu.

Abstract: Wooden objects with heritage value, both exposed to the external environment and the enclosed spaces are often exposed to both fungal and bacterial attack. Degradation often occurs while no visible exterior but may have irreversible effect. Usually, the bacteria that attack the wood does not have action against of human body. However, there have been reported some cases of the patients with infections caused by phytopathogenic bacteria. The aim of the study was to test active compounds of the hydroethanolic extract of Juglans nigra against phytopathogenic bacteria with implication also on the human body. The active compounds with antibacterial activity have been identified and separated by thin-layer chromatography (TLC). MCI of the hydroethanolic extract of Juglans nigra had values between 15,625 pg / mL and 250 pg / mL.In conclusion, we can say that these extracts can be used successfully againstbacterial pathogens.

Keywords: Juglans nigra, antibacterial compounds, Gram positive and Gram negative

bacteria

Introduction

Both Gram-positive and Gram negative bacteria were reportated as having degrade wood. Favoring factors colonization in wood polymer are numerous, these being found in both water and soil and in the air. Bacillus spp. are a diverse group of bacteria widely distributed in soil and aquatic medium, implications for wood degradation. They are aerobic Gram positive bacteria, endospore-forming in the form of rods being the most common soil microorganisms. Due to their ability in forming the spores, they are resistant to a range of varying conditions of the environment and they adapt easily to different habitats. On the basis of 16S rDNA sequence analysis, have been identified Bacillus subtilis, В. amyloliquefaciens, В. pumilus and

В. atropbaeus. Recently, it was reported that B. pumilus is the second most prevalent species Bacillus. This bacteria is highly resistant to extreme environmental conditions, such as reduced availability of nutrients or lack thereof, dryness, radiation, H202 and chemical disinfection. Some strains of B. pumilus with habitat in soil colonize the roots of plants having an ecological role inproduce compounds for fungal and bacterial antagonists. B. pumilus has a role to ensure

M A R I S I A, XXXV, p. 41-48