Marisia - Maros Megyei Múzeum Évkönyve 35/2. (2015)
Botany
MICROPROPAGATION OF BLACKBERRY CULTIVARS - PERSPECTIVES FOR REPUBLIC OF MOLDOVA Mariana LOZINSCHII1, Nina CIORCHINÄ1, Tatiana CALALB2 1 Botanical Garden(Institute) of Academy of Science of Moldova, 18 Padurii Street Chisinau, MD-2002 diaconm@mail.ru, ninaciochina@mail.ru 2 State University of Medicine and Pharmacy „Nicolae Testimiteanu”, Bd. §tefan cel Mare §i Sfant, 165 Chisinau, MD-2004, tatiana.calalb@usmf.md Abstract: The given paper describes the development of cultivation conditions of some blackberry thornless cultivars of Rubus lacinatus, R. canadensis, R. fruticosus species in vitro culture such as: Thornless evergreen, Arapaho, Polar, Remontana, Thornfree, Smothstem, Loch Ness. The sterilization protocol of the original material was assessed. The basal media Murashige & Scoog 1962 (MS) with the addition of 30 g/1 sugar was created for the in vitro initiation and development. The best medium for multiplication is MS 100%, supplemented with a growth regulator, 6-benzylaminopurine (BAP) 0.5 mg/1. And other growth regulathors were utilized in the micropropagation process like: Indolylbutyric Acid (IBA), and Acid Naphthyl Acetic (NAA). Keywords: cultivar, in vitro, initiation, blackberry, micropropagation, culture medium, growth regulator. Introduction Plant biotechnology is one of the main achievements of the twentieth century, with a particular role in developing modern agriculture and horticulture. In vitro micropropagation is a branch of plant biotechnology which represents a collection of plant multiplication methods using in vitro cell cultures, plant tissues and plant organs. The use of this technique permits to considerably increase the efficiency of multiplication of different species, and it is also a method to free the pathogens from the seedling material. Micropropagation of shrubs was investigated by many reasearchers who explored the initiation, multiplication and acclimation of a new variety of thornless blackberry 'Cacanska bestrna' [3, 6]. At the Botanical Garden (Institute) of Academy of Science of Moldova (ASM), the micropropagation and multiplication of blackberry cultivars was started in 2010 and there were obtained good results in the initiation, multiplication, rootedness, ex vitro adaptation of some cultivars [4, 5]. At Fruit Research Station of Cluj, an effective method was obtained, which combines two phases, a direct ex vitro rooting and acclimation of blackberry shoots in the hydroculture flotation [2]. Based on hydroponic flotation technology and ex vitro acclimation of Loch Ness blackberry [1]. The present work reveals some problems regarding the multiplication of valuable varieties of blackberry using biotechnology methods. The aim of the research brought up in this paper M A R I S I A, XXXV, p. 9-16
