Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)
Investigated bone samples and methods
Y-chromosomal haplogroup analyses Y-chromosome haplogroups were determined using the SNV markers described in Karmin, Saag et al. (2015), Poznik, Xue et al. (2016) and Rootsi, Behar et al. (2013) Mitochondrial haplogroup analyses Because of the higher copy number of mitochondrial genomes present in cells, we obtained good coverage depth and percent of mitochondrial genome covered for all samples. The same data were used to determine the percentage of mitochondrial genome with less than ten-fold coverage. We used the PhyloTreeMT (Buildló) to infer the mitochondrial haplogroups (Van Oven and Kayser 2009). Each marker was individually visualized and visually verified to avoid the possibility of a variant calling error. Statistical analysis of the relationship between Béla III and skeleton II/52_3 based on A-STR markers With regards to the A-STR marker alleles, allele frequencies and the alleles European and Hungarian mutation frequency, we gave the probability of family relations with the following method (Dr Gábor Tusnády): Investigated persons are supposedly members of the same royal family, but membership is not necessary. We know from other sources that persons B and A are couples, B is the husband, a King, A is the wife, a Queen. C is an infant, other persons are men but 9 are female. The persons C,G,H,3,7,9,0,8 are supposed to be descendants of the couple B, A. 233
