Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

Investigated bone samples and methods

d. The

length of individual reads (sequenced DNA strands) are the following: D2S441, D2S1338, D19S1338 = 151 bp, D3S1358, D7S820 = 142 bp.

Table 23. Chromosome regions and PCR primers.

Chr

A-STR

Genome region (bp)

PCR primer forward

PCR primer reverse

2pl3.3

D2S441

2:68239063-68239103

AGGAACTGTGGCTCATCTATG

TTCACTCTCCTTCCCAAATGTTTA

2q35

D2S1338

2:218879568-218879718

CATAATCATGAGTTATTCAGTAAGTTAAAGG

GAGCCAGTGGATTTGGAAAC

3p21.32

D3S1358

3:45582186-45582336

GGCATCTCTTATACTCATGAAATCA

CCCACTGCAGTCCAATCTG

7q21.11

D7S820

7:83789519-83789619

GTAATTAAATGTTTACTATAGACTATTTAGTGAGAT

GGTATGATAGAACACTTGTCATAGTT

19ql2

D19S433

19:30417112-30417212

GCACCCATTACCCGAATAAAAATC

GGCTGCAAAAAGCTATAATTGTAC

Evaluation of the data was performed using the Burrows-Wheeler Alignment Tool (BWA) software. Description of the software: “BWA is a software package for mapping low-divergent sequences against a large reference genome, such as the human genome (HG19). It consists of three algorithms: BWA-backtrack, BWA-SW and BWAMEM. The first algorithm is designed for Illumina sequence reads up to 100 bp, while the remaining two for longer sequences ranged from 70 bp to 1 Mbp (Lee and Durbin, 2010)”.

We compared the sequence-order of alleles determined during sequencing to data gathered from the following database: STRbase: tandem repeat DNA internet database (Butler JM and Reeder DJ 1997; Ruitberg et

al.

2001).

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