Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)

Investigated bone samples and methods

7. Digestion

of supernatant in Proteinase K (200pg/ml) solution at 56°C for a whole night (with shaking: 300rpm). A second analysis on the remaining bone dust, the same manner as with the first isolation.

8. DNA

isolation with a Qiagen MinElute Gel Extraction Kit in an Elutio 40

pl IxTE puffer.

9. Control

PCR amelogenin on DNA with controls of known concentration.

STR-marker detection with PCR method

According to experience, the success of the detection of A-STR markers depends partly on the method of detection applied, thus the detection kit or DNA sequencing method, and how the results are read.

The Göttingen

and

Budapest-1 laboratories used the following detection kits to detect A-STR and Y-STR markers:

Göttingen laboratory

(Seidenberg,

Dr

Hummel)

For the autosomal STR-marker investigations the following, generally available detection kits were used: (1) Heptaplex miniSTR assay, Seidenberg

et

al. (2012);

(2) a Decaplex miniSTR assay. Fehren- Schmitz et

al.

(2015); (3) Investigator ESSplex SE plus and Investigator ESSplex SE

QS

(Qiagen) a 0.5-5 pl with prior PCR amplification of the DNA sample.

The Y-chromosome STR-marker analyses were performed using the following kits: Powerplex Y

kit

(Promega) and the lab-internal decaplex Y-miniSTR kit (we do not show primer sequences at this time).

Budapest -1 laboratory (Dr Tudit Olasz,

Dr

Orsolya

Csuka)

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