Miklós Kásler - Zoltán Szentirmay (szerk.): Identifying the Árpád Dynasty Skeletons Interred in the Matthias Church. Applying data from historical, archaeological, anthropological, radiological, morphological, radiocarbon dating and genetic research (Budapest, 2021)
CHAPTER SEVEN – Genetic investigations
As we can see, the isolated DNA was suitable for the amplification of A-STR markers (determining the genetic fingerprint). This amplification was initially performed using the Heptaplex kit (Seidenberg et al. 2012). This kit is capable of detecting 6 A-STR markers and the amelogenin marker (for gender determination). For preliminary orientation, Figure 32 shows the compiled results of six A-STR markers; this gives us an overview of the detectability of the chosen markers and of the artifacts that were generated. The result of the modified DNA isolation also allows for the conclusion that the DNA sample thus obtained can also be used for next generation sequencing (NGS). Figure 32. Six A-STR localizations and the amelogenin profile detected on the bone samples of Béla III and skeleton II/52_3 using the Heptaplex kit. The identical markers are marked by a red line (Göttingen laboratory). Additionally, the figure contains alleles which are not identical in the two bone samples (Seidenberg et al. 2012). 130
