A Móra Ferenc Múzeum Évkönyve, 1968. (Szeged, 1968)

Gallé, László: The xerothermic lichen species cladonia magyarica VAIN

we have used the gonidia of the Cladonia magyarica thalluses originating from the F. KISS wood and BILISICH wood beside Szeged-Ásotthalom. The inoculation was carried out with the method used in the Geneva school an followed by JA AG, too. As culture medium we have used a culture medium with glucose prepared with one-third of DETMER's agar-agar, sand soil with glucose from the F. KIS wood, 2/1 of DETMER's solution with glucose, and DETMER's modified solution with glucose. The inoculated cultures were put into a culture case placed before a window giving a diffuse light and looking on the direction NNE. In the case a temperature of 16-18° С was assured. The inoculation of cultures took place on February 21st. For a complete month there has not occured - according to cur macroscopic observation - any visible change. Between March 20th and 22nd some vaccines took place. At the bottom of a culture with DETMER's modified culture fluid and on the surface of more nutrient substrata some dots of 0,5 mm diameter have appeared. The thalluses continued developing gradually but very slowly. On June 19th the size of cultures was of a diameter of 5-7 mm. From the cultures grown large enough, on April 19th and May 6th we made transmissions by inoculation to different culture media. Erlenmeyer's retorts containing the vaccines were kept partly in a diffuse, partly in a direct sunlight. With these series we have wished to investigate the to influence of the different culture media and of the luminous intensity. The cultures grown in DETMER's sugared culture medium from the F. KISS wood have shown round cultures with a size of 5 mm. That consisted of fine small clods showing in some places knobs, elswhere dimples. The knobs were placed as if their centre had developed alga groups along concentric circles starting from the same centre. The middle of culture was thicker, it protruded more than the peripheric part that seemed to be thinner. Its colour is yellowish-green (Code des Couleurs: Vert No. 302), of a somewhat wet surface without being bright. The gonidia dissolved in a sugared soil concoction from the BILISICH wood have given small thalluses of a less regular shape but of a much finer granulation, and therefore of a smoother surface. Their colour was more yellowishgreen (Code des Couleurs: Vert No. 307), the surface wetter and brighter. Also the external picture of the thalluses grown in culture media non-sugared was similar to those described above, the small thalluses have however formed a smaller and thinner cover. The granulation of the thalluses developed in solid culture media and visible to the naked eye supposedly is a cell group fragmentated from a mother cell each. The gonidia haven't reveal any difference in direct and diffuse lights as there wasn't given any difference by DETMER's sugared solution and a sugared soil concoction. The want of sugar has, however, retarded the development strongly. The results of the macroscopic investigations of cultures are: (1) The presence of glucose is accelerating the development; without glucose, however the intensity of development is decreasing. (2) The use of a direct or diffuse sunlight is giving no difference at gonidia coming from the same plant and locality and inoculated into the same culture medium. 245