A Móra Ferenc Múzeum Évkönyve, 1968. (Szeged, 1968)

Gallé, László: The xerothermic lichen species cladonia magyarica VAIN

agar. A half part of the culture media and culture fluids was given with 2 percent glucose. The culture media have been made as follows: DETMER's standard solution was diluted with distilled water in the above-mentioned ratio, then we have given 2 percent agar-agar to it and the whole was boiled in an autoclave, in Erlenmeyer's test-tubes closed with wadstoppers. The boiling culture medium, in a fluid state, was filtered through a cotton-wool filter and distribuer into eprouvettes, resp. smaller Erlenmeyer's test-tubes. The glucose in the quantity necessary to the culture medium has been given to the culture fluid after being boiled but before being filtered. The eprouvettes and Erlenmeyer's test-tubes ware sterilized, closed with wadstoppers, in an autoclave, held 20 minutes long under a pressure of one atmosphere, about in 110-120 °C. We let the eprouvettes get cold in an inclined state, gettling so slanting culture medium surfaces. For making pure cultures, we have used soil concoctions, as well. The sand was boiled with distilled water - calculating two parts of water to one part of sand - 10-15 minutes long, in a temperature between 90-100° C. After discharging the solution from the cooled and settled concontion, we have filtered it through a filtering paper and used that instead of DETMER's solution. The soil concoction was used also with agar-agar. 2 percent of agar-agar was applied in the soil-concoction, with glucose or without sugar. The culture fluids and culture media used at the second series of experiments have been: DETMER's solution of 2/1 concentration, without agar-agar, with 4 p. c. glucose, DETMER's solution of 1/3 concentration, with 2 p. с agar-agar, with 4 p. с glucose, A soil-concoction made of dry sand from the F. Kiss wood, with 2 p. с agar-agar, 4 p. v. glucose, DETMER's solution with JAAG's modification, without agar-agar, with 4 p. с glucose. The composition of DETMER's modified solution is: H

2

0 1000 g KH

2

P0

4 0,25 g NH

4

C1 0,650 „ KCl 0,25 „ CaCl

2 0,675 „ Mg S0

4 0,24 „ FeCl

3 (1 p. c.) . . . . 1 drop. In the first series of experiments R. CHODAT's method of pure breeding was applied (CHODAT, 1913. p. 193). A piece of the thallus squamae of Cladonia magyarica was rinsed clean with distilled water before being inoculated. Then it was squashed in a drop of distilled water, macerated with a lancet. Thus we have obtained a mass consisting of gonidia and hyphae. The inoculation took placewith a heated platinum needle. The needle was dipped into a waterdrop containing the hyphagonidium mass and the materialsticked to the neddle was inoculated into the culture medium, resp. the culture fluid. The substance inoculated in this way is not clean, as yet; besides gonidia and algae, also a lot ot bacteria, penicillia, hyphae have got into the culture medium. From the infecting organisms - particulary in a culture medium 16 A Móra F. Múzeum Évkönyve 241