Hidrológiai Közlöny, 2015 (95. évfolyam)
2015 / 5-6. különszám - LVI. Hidrobiológus Napok előadásai
95 Johnson, P.W., & Sieburth, J. McN. (1979) Chroococcoid cyanobacteria in the sea: A ubiquitous and diverse phototrophic biomass. Limnol. Oceanogr. 24: 928-935. Kolber, Z.S., Van Dover, C.L., Niederman, R.A. & Falkowski, P.G (2000) Bacterial photosynthesis in surface waters of the open o- cean. Nature 407: 177-179. Kolber, Z.S., Plumley, F.G, Lang, A.S., Beatty, J.T., Blankenship, R.E., Van Dover, C.L., et al. (2001) Contribution of aerobic pho- toheterotrophic bacteria to the carbon cycle in the ocean. Science 292: 2492-2495. Koblízek, M., Masín, M., Josephine, R., Poulton, AJ. & Prásil, O. (2007) Rapid growth rates of aerobic anoxygenic phototrophs in the ocean. Environ. Microbiol. 9: 2401-2406. Koblízek, M, Mlcouková, J, Kolber, Z. & Kopecky, J. (2010) On the photosynthetic properties of marine bacterium COL2P belonging to Roseobacter clade. Arch. Microbiol. 192: 41—49. Lami, R., Cottrell T. M., Ras J., Ulloa O., Obemosterer I., Claustre H., Kirchman D. L. & Lebaron P. (2007) High Abundances of Aerobic Anoxygenic Photosynthetic Bacteria in the South Pacific Ocean. Appl. Environ. Microbiol. 73 (13) p. 4198-4205. Masín, M., Zdun, A., Ston-Egiert, J., Nausch M. (2006) Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea. Aquat. Microb. Ecol. 45: 247-254. Maclsaac, E. A. & Stockner, J. G (1993) Enumeration of phototrophic picoplankton by autofluorescence. In Kemp, P.F., B. F. Sherr, E. B. Sherr & J. J. Cole (eds), Handbook of methods in aquatic microbial ecology, Lewis Publishers, Boca Raton, Ann Arbor, London, Tokyo, pp. 187-197. Schwalbach M. S. & Fuhrman J. A. (2005) Wide-ranging abundances of aerobic anoxygenic phototrophic bacteria in the world ocean revealed by epifluorescence microscopy and quantitative PCR. Limnol. Oceanogr., 50: 620-628. Medová, H., Boldareva, E.N., Flrouzek, P., Borzenko, S.V., Namsaraev, Z.B., Gorlenko ,V.M., Namsaraev, B.B., & Koblízek, M. (2011) High abundances of aerobic anoxygenic phototrophs in saline steppe lakes. FEMS Microbiol. Ecol. 76: 393^100. Shiba, T. (1991) Roseobacter litoralis gen. nov., aerobic pink- pigmented bacteria which contain bacteriochlorophyll a. Syst. Appl. Microbiol. 14: 140-145. Sieracki, M. E., Gilg, I.C., Thier, E.C. & Poulton, N.J. (2006) Distribution of planktonic aerobic anoxygenic photoheterotrophic bacteria in the northwest Atlantic. Limnol. Oceanogr. 51: 38-46. Vörös, L., Gulyás P. & Németh,J. (1991) Occurrence, dynamics and production of picoplankton in Hungarian shallow lakes. Int. Rev. Hydrobiol. 76: 617-629. Waidner L. A. & Kirchman D. L. (2007) Aerobic Anoxygenic Phototrophic Bacteria Attached to Particles in Turbid Waters of the Delaware and Chesapeake Estuaries. Appl. Environ. Microbiol. 73 (12): 3936. Waterbury, J.B., Watson, S.W., Guillard, R.R.L. & Brand L.E. (1979) Widespread occurrence of a unicellular, marine, planktonic cyanobacterium. Nature 277:293-2. Phototrophs in near-infrared - first data on the occurrence of aerobic anoxygenic bacteria in Hungarian shallow lakes Nóra Tugyi', Lajos Vörös', Emil Boros', Tamás Felföldi2, Károly Márialigeti2 and Boglárka Somogyi' 'MTA ŐK Balaton Limnological Institute, Tihany 2Eötvös Loránd University, Budapest Abstract: It has been discovered only during the last decade, that bacteriochlorophyll o-containing bacteria, the so-called aerobic anoxygenic phototrophs (AAP) - which use near-infrared light to gain energy - are quite abundant in the plankton of the upper oceans and thought to be important players in oceanic carbon cycling. These microorganisms are barely studied in ffeshwaters, notwithstanding their presence in the oxic layer has been justified during the last years in Central European mountain and North European humic lakes. Identification of AAP cells requires near-infrared detection (800-900 nm).Therefore, the aim of this research was to study the occurrence of AAP in Hungarian shallow lakes and to assess their importance in the microbial community (with special respect to the photoautotrophic picoplankton and heterotrophic bacterioplankton). AAP was studied using an epiflourescence microscope (Olympus BX51) equipped with an infrared camera (Olympus XM10-IR) in summer 2014. As a result, AAP was found in high abundance in the studied lakes: their presence was verified in Lake Balaton, in soda lakes of Seewinkel, in fishponds of Somogy County and shallow turbid or humic soda pans of the Danube-Tisza Interfluve. Highest abundances (~ 100 million cells per milliliter) were detected in more productive waters. A positive correlation was found between AAP abundance and phytoplankton biomass (chlorophyll a). The contribution of AAP to total bacterial abundance ranged between 4 and 40% which indicated the important role of these microroganisms in carbon and energy flow. Key words: aerobic anoxygenic phototrophs (AAP), photoheterotrophic lifestyle, shallow lakes, infrared microscopy.
