Hidrológiai Közlöny, 2014 (94. évfolyam)

2014 / 1. szám - Petruska Fanni - Geda Orsolya - Resetár Anna - Ajtay Kitti: A lemna-minor, a Lemna trisulca és a Wolffia arrhiza tenyészetek cilindrospermopszinnal szembeni érzékenységének összehasonlító vizsgálata

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HIDROLÓGIAI KÖZLÖNY 2014. 94. ÉVF. 1. SZ.

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Comparative analysis of the sensitivities of different Lemnaceae clones to cylindrospermopsin containing cell

free extracts of Aphanizomenon ovalisporum.

Fanni, Petrusha - Orsolya, Geda - Anna, Resetár - Kitti, Ajtay University of Debrecen Faculty of Science and Technology, Department of Botany, H- 4032. Debrecen, Egyetem tér 1.

Abstract: Nowadays the expansion of the areas of bloom forming cyanobacteria with cyanotoxin producing potenciál was confirmed.

One of the serious impacts of eutrophication of aquatic ecosystems can be the disappearance of the senstivive submerged macrophytes and the shift to a phytoplankton-dominated state. Recently, an increasing number of studies is concerned with the biological effects of cylindrospermopsin (CYN), -a cyanotoxin with protein synthesis inhibitory effect-, but in comparison to other cyanotoxins, there are only a few data of physiological and biochemical alterations caused by CYN in plants. We compared the effects of CYN containing (0-10 gg ml-l) whole-cell extract of A. ovalisporum on the chlorophyll-, protein contents and pyrogallol peroxidase activities of different clones of Lemna minor, Lemna trisulca and Wolffia arrhiza. Based on chl-a and chl-A contents the most sensitive clone was one of the Lemna minor culture lines collected in Monostor- pályi, Hungary. At 10 pg ml CYN decreased chlorophyll, but increased protein contents and peroxidase activities were measured in Lemna species (in both, minor and trisulca). In Wolffia these stress reactions were characteristic at 0,1-1 pg mlCYN concentration range. Our results are in good agreement with results of Kinnear et al. (2007, 2008), Jámbrik et al. (2009) and Szigeti et al.(2010), who measured decreased chlorophyll content and elevated protein content in different CYN treated aquatic plant species. One putative mechanism of CYN action may be the induction of oxidative stress similar to microcystins (Kinnear et al., 2008, M-Hamvas et al. 2010, Prieto et al. 2011, Máthé et al. 2013a), which was further proved by our results concerning elevated peroxidase activities in CYN treated Lemnaceae plants.

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A kézirat beérkezett: 2014. január 15-én