Hidrológiai Közlöny 2012 (92. évfolyam)

5-6. szám - LIII. Hidrobiológus Napok: „A hidrobiológia szerepe a víz-stratégiákban” Tihany, 2011. október 5–7.

91 Hiripi, L„ Nagy, L„ Kalmár, T„ Kovács, A. & Vörös, L. (1998) Insect (Locusta migratoria migratorioides) test monitoring the toxicity of cyanobacteria. Neurotoxicology 19: 605-608. Holte, HR, Eriksen, S, Skulberg, O. & Aas, P. (1998) The effect of water soluble cyanotoxin(s) produced by two species of Anabaena on the release of acetylcholine from the peripheral cholinergic nervous system of the rat airway Environ. Toxicol. Pharmacol. 5: 51-59. Kiss T., Vehovszky, A., Hiripi, L., Kovács, A. & Vörös, L. (2002). Membrane effects of toxins isolated from a cyanobacterium, Cylindrospermopsis raciborskii, on idenfified molluscan neurones. Comp. Biochem. Physiol. C. 131: 167-176. MacKay, A. & Gelperin, A. (1972) Pharmacology and reflex responsiveness of the heart in the giant garden slug, Umax maximus Comp. Biochem. Physiol. A: 43, 877-896. Meldahl, A.S.; Aas, P. & Fonnum, F. (1996) Extract of the marine alga Prymnesium patelliferum induces release of acetylcholine from cholinergic nerves in the rat bronchial smooth muscle. Acta Physiol. Scand. 156: 99-107. Singh, S., Kate, B.N., Banerjee, U.C. (2005) Bioactive compounds from Cyanobacteria and Microalgae: an Overwiev. Critical reviews in Biotechnology. 25: 73-95. Van Dolah, F.M. & Ramsdell, J.S. (2001) Review and assessment of in vitro detection methods for algal toxins J.Aquatic Int. 84:1617-1625 Vasas, G. Borbély, P. & Nánási, P.P. (2010). Alkaloids from Cyanobacteria with Diverse Powerful Bioactivities. Mini-Reviews in Medicinal Chemistry 10: 946-955. Walker, R.J. Isolation and in situ heart preparation of the snail, Helix aspersa. (1968) In: Kerkut, G.A., (Ed). Experiments in Physiology and Biochemistry 1, Academic Press, London, 1968, pp. 331-342. Walker, R.J., Brooks, H.L. & HoldenDye, L. (1996) Evolution and overview of classical transmitter molecules and their receptors Parasitology 113: S3-S33. . Pharmacological characterization of the bioactive effects of cyanobacterial extracts isolated from the lake Balaton, Hungary Vehovszky, A., Kovács, W. A., Szabó, H., Győri, J., Farkas, A. Balaton Limnological Research Institute, HAS, Tihany, H-8237 Hungary, Abstract: The responses of the central neurons (Helix pomatia, Lymnaea stagnalis) and the isolated Helix heart were characterized after application of extracts made from cyanobacterial isolates (Cylindrospermopsis raciborskii ACT strains) collected during natural blooms from Lake Balaton (Hungary) by the Balaton Limnological Research Institute, Tihany. On identified neurons in Helix pomatia and Lymnaea stagnalis central nervous system the crude extracts of both the ACT 9502 and ACT 9505 strains evoked acetylcholine agonist and antagonist effects suggesting some anatoxin-a like neuroactive components acting on nicotinergic acetylcholine receptors in the CNS. The above suggestion is confirmed by our results obtained on the isolated Helix heart: similar contractions and inhibition of the spontaneous activity were evoked by the crude ACT 9505 extracts and the anatoxin-a solution. Both of these effects are probably evoked by direct modulation of the electrical or contractile system of the heart, as ACh evoked opposite effects (heart relaxation). These data, therefore, suggest the involvement of some non-cholinergic mechanisms acting during the cyanobacterial effects on heart. Keywords: Cylindrospermopsis, Helix, Lymnaea, acetilkolin, anatoxin-a. Antal, O., Mariann Karisztl-Gácsi, M., Farkas, F., Kovács, W.A., Acs, A., Törő, N„ Kiss, G., Saker, ML., Győri, J., Bánfalvi, G. & Vehovszky, A. (2011). Screening the toxic potential of Cylindrospermopsis raciborskii strains isolated from Lake Balaton, Hungary. Toxicon 57: 831-840. Araoz, R., Molgo, J. & Marsac, N.T. (2010) Neurotoxic cyanobacterial toxins Toxicon 56: 813-828. Buckett, K. J. Dockray, G. J. Osborne N. N. & Benjamin P. R. (1990) Pharmacology of the myogenic heart of the pond snail Lymnaea stagnalis JNeurophysiol 63: 1413-1425. Bernard, C, Harvey, M., Briand, J.F., Bire, R., Krys, S. c£ Fontaine, J.J. (2003) Toxicological comparison of diverse Cylindrospermopsis raciborskii strains: Evidence of liver damage caused by a French C-raciborskii strain Env. Toxicol. 18: 176-186. Elliott, C.J.H., Stow, R.A. & Hastwell, C. (1992) Cholinergic interneurons in the feeding system of the pond snail Lymnaea stagnalis 1. Cholinergic receptors on feeding neurons Phil. Trans.Roy.Soc. Lond. B. 336: 157-166. Farkas A., Kovács W.A., Gácsi M., Győri J., Saker, L.M. & Vehovszky, A. (2007) Toxicity assessment of cyanobacteria by the Artemia salina acute toxicity assay (in Hungarian.; Cianobaktériumok toxikusságának vizsgálata a sórák (Artemia salina L.) akut toxicitási teszt alkalmazásával. Hidrológiai Közlöny 87: 28-30. Gordon, R.K., Gray, R.R., Reaves, C.B., Butler, D.L. & Chiang, P. K. (1992) Induced release of acetylcholine from guinea pig ileum longitudinal muscle myenteric plexus by anatoxin-a J. Pharmacol. Exp. Therapeut. 263: 997-1002. Hill, R.B. & Welsh, J.H. (1966) The major inhibitory transmitter on molluscan hearts is Ach. Heart circulation and blood cells. In: Wilbur, K.M. and Yonge, C.M., Editors, Physiology of Mollusca 2, Academic Press, New York, 1966. pp. 125-174.