Hidrológiai Közlöny 2009 (89. évfolyam)

6. szám - L. Hidrológiai Napok: "A hazai hidrobiológia ötven éve" Tihany, 2008. október 1-3.

125 M-Hamvas M., Jámbrik K, Máthé Cs., Vasas G., Bácsi I., Beyer D., és Borbély Gy. (2007) A mikrocisztin-LR (cianotoxin) hatásai különböző vízinövényfajokra. A IV. Kárpát-medencei Környezettudományi Konferencia kiadványa. Mitrovic S.M., Allis, 0., Furey, A., James, K.J. (2005) Bioaccumulation and harmful effects of microcystin-LR in the aquatic plants Lemna minor and Wolffia arrhiza and the filamentous alga Chladophora fracta. Ecotoxicology and Environmental Safety. 61: 345Padisák J (1997) Cylindrospermopsis raciborkii (Woloszynska) Seenayya et Subba Raju, an expanding, highly adaptive cyanobacterium: worldwide distribution and review of its ecology. Arch. Hidrobiol. /Suppl. 4:563-593. Pflugmacher S. (2004) Promotion of oxidative stress in aquatic macrophyte Ceratophyllum demersum during biotransformation of the cyanobacterial toxin microcystin-LR. Aquatic Toxicology. 70: 169Preussel K Stuken A., Wiedner C., Chorus I. and Fastner J. (2006) First report on cylindrospermopsin producing Aphanizomenon flos -aquae (Cyanobacteria) isolated from two Germán lakes. Toxicon 47(2): 156-162. Reisner M., Carmeli S., Werman M., Sukenik A. (2004) The cyanobacterial toxin cylindrospermopsin inhibits pyrimidine nucleotide syinthesis and alters cholesterol distribution in mice. Toxicological Sciences 82: 620-627. Shaw G. R., Sukenik A., Livne A., Chiswell R. K, Smith M. J., Seawright A. A., Norris R., L., Eaglesham, G. K, Moore, M. R. (1999) Blooms of the hepatotoxic cyanobacterium, Aphanizomenon ovalisporum (Forti) in newly constructed lakes, Queensland, Australia. Environ. Toxicol. 14: 167-177. Schembri M.A., Neilan B.A. and Saint C.P.(2001) Identification of genes implicated in toxin production int he cyanobacterium Cylindrospermopsis raciborskii. Environ Toxicol 16 (5): 413-421. Schlereth A., Becker C., Horstmann C., Tiedemann J., Müntz K. (2000) Comparison of globulin mobilization and cysteine proteinases in embryogenic axes and cotyledons during germination and seedling growth of vetch (Vicia sativa L.). Journal of Experimentál Botany 51 (349): 1423-1433. Tumer P.C., Gammie A.J., Hollinrake K, Codd G.A. (1990) Pneumonia associated with contract with cyanobacteria. brit. Med. J. 300, 1165-1175. Vasas G., Gásprár A., Surányi G., Batta G., Gyémiánt Gy., M. Hamvas M„ Máthé Cs., Grigorszky I., Molnár E„ Borbély G. (2002) Capillary Elecfiophoretic assay and purification of cylindrospermopsin, a cyanobacterial toxin from Aphanizomenon ovalisporum by plánt test (Blue-Green Sinapis Test). Analytical Biochemistry 302: 95Vasas G., Gáspár A., Páger C., Surányi G., Máthé C., M-Hamvas M., Borbély G. (2004) Analysis of cyanobacterials toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis. Electrophoresis 25: 108-115. White S.H., Duivenvoorden L.J., Fabbro L.D., Eaglesham G.K (2007) Mortality and toxin bioaccumulation in Bufo marinus following exposure to Cylindrospermopsis raciborskii cell extracts and live cultures. Environmental Pollution 177: 158-167. Wilson C. M. (1975) Plánt nucleases. Annu. Rev. Plánt Physiol. 26: 187-208. Investigation of sensitivity of aquatic plants to the cylindrospermopsin K. Jámbrik, M. M-Hamvas, Cs. Máthé, D. Beyer, I. Bácsi, G. Koncz, Sz. Tóth, Gy. Surányi and Gy. Borbély University of Debrecen, Department of Botany, H-4010, Debrecen, Egyetem tér 1. Abstract: Cyanobacteria can produce a wide rangé of bioactive and toxic substances which can be harmful to wildlife and humans. One of these toxins is cylindrospermopsin (CYN), a cyanobacterial guanidine alkaloid hepatotoxin and protein synthesis-inhibitor. The aim of this study was to compare the effects of CYN containing cell free extracts of Aphanizomenon ovalisporum (BGSD-423) culture on three aquatic plánt species. In our study during exposure of Ceratophyllum to CYN in a concentration of 2,5; 5 pg ml'

1 an elevation of protease and ssDNase enzyme activity was detected after 19 days on the activity gels. Lemna minor was sensitive to CYN as well. The growth parameters and chlorophyll content were significantly reduced by 10, 20 |ig ml"

1 CYN. Enzyme activity gels (ssDNase and protease) showed significant increase of enzyme activities under the influence of CYN. 20 pg ml"

1 CYN concentration induced appearance of new protease izoenzymes (32, 35, 38, 58 kDa). Wolffia arrhiza plants were the most sensitive to CYN. Growth (as weight and frond number) and chlorophyll content were significantly reduced after 5 days of exposure to concentrations of 5-20 pg ml"

1. The level of ssDNase activity would be higher and the isoenzyme patterns of protease enzymes showed characteristic changes in CYN treated plants compared to contol ones. Several protease-isoenzymes (43, 55, 60, 70 kDa) were induced in the CYN treated plants. Keywords: Aphanizomenon ovalisporum, cylindrospermopsin, Wolffia, Lemna, Ceratophyllum. (Klossy Irén alkotása)