Hidrológiai Közlöny 2004 (84. évfolyam)
5-6. szám - XLV. Hidrobilógus Napok „Vizeink hosszú idejű változásai” Tihany, 2003. október 1–3.
167 Irodalom: 2., Vasas G., Padisák J„ M-Hamvas M., Máthé Cs., Molnár E„ Surányi Gy., Grigorszky I., Borbély G. (2002) Hidr. Közlöny 80. 380-382 1., Vasas G., Gáspár A.,Surányi Gy., Gyémánt Gy., M-Hamvas M., 3 . M-Hamvas M., Máthé Cs., Molnár E., Vasas G., Grigorszky 1., Máthé Cs., Grigorszky I., Molnár E., Borbély G, (2002) Anal. Bio- Borbély G. (2002) Aquatic Toxicology 62 1-9 ehem. 302. 95-103 4., Vasas G., Gáspár A., Páger Cs., Surányi Gy., Máthé Cs., M-Hamvas M., Borbély G., Electrophoresis közlésre elfogadva. CH, CH, H,N ^VlH MCYST-LY MCYST-HilR CH,0 O [D-Ser 7] MCYST-EE(OMe) CH, CH, 1. ábra Az azonosított mikrocisztinek képlete Abstract: Cyanobacteria may induce environmental problems in fresh water and in marine environments. They are producing a range of bioactive compounds e.g. peptides, polyketides, alkaloids and lipopolysaccharides. Some of these compounds are neuro- or hepatotoxines. Microcystin is a hepatotoxic heptapeptide produced by several species of the genera Microcystis, Anabaena , Nostoc, and Oscillatoria. Microcystin have more than 65 stuctural isomforms, having the common stucture cyclo(Adda-D-Glu-Mdha-D-Ala-L-X-D-MeAsp-L-Z), where X and Z are available L-aminoacids, Adda is 3-amino-9-methoxy-2,6,8,-trimethyl-10-phenyl-4,6-decadienoic acid, D-MeAsp is 3-methyIaspartic acid and Mdha is Af-methyl-dehydroalanine. From water blooming Kis-Balaton reservoir, Hungary we isolated several microcystins (Fig. 1). The isolation and the chemical identification of those compounds was obtained by HPLC separation, MS, NMR and UV spectroscopy. The chromatographic separation and the biological characterization of microcystins were performed with the help of a plant test (mustard), namely the Blue Green Sinapis Test (BGST).
