Hidrológiai Közlöny 1998 (78. évfolyam)

4. szám - Fülöp István Antal–Józsa János: A neruális hálózatok világa

297 Köszönetnyilvánítás Ez a munka a Környezetvédelmi és Területfejlesztési Minisztérium, a Miniszterelnöki Hivatal, és az OTKA (T022988) támogatásával készült. 5 5 5 s; <3 LüJ Ll JL ü THHWp imnísp lOSBp HRY WSBsp Pigment keverék Cylindrospermopsis raciborskji Microcyslts aemginosa i u ily U Selenastrum caprlcomutum Nttzschia palea Fitoplankton Minta (Tihany) 1. ábra. Autentikus pigment keverék, tiszta algatenyészetek és fitoplankton minta HPLC-s kromatogramja (%) 80 60 40 20 Abstract: Keywords: 3. ábra. A fitoplankton pigmentdiverzitása (p) és faj-egyed diverzitása (sp) a Balatonban és a Kis-Balatonban (H = Shannon diverzitás, J egyenletesség). Irodalom Carpenter S.R.. ElserMM., ElserJJ. (1986) Chloropyll production, degradation and sedimentation: Implication for paleolimnology, Limnol. Oceanogr. 31,112-124. Jacobsen T.R. (1982) Comparison of chlorophyll-a measurements by fluorometrie, speetrophotometrie and high pressure liquid chromatographic methods in aquatic énvironments. - Arch. Hydrobiol. Beih. Ergebn. Limnol. 16,35-45. Leavitt P.R., Carpenter S.R., Kitchell J.F. (1989) Whole-lake experiments: the annual record of fossil pigments and zooplankton. Limnol Oceanogr. 34, 700-717. Leavitt P.R., Carpenter S.R. (1990) Regulation of pigment sedimentation by photo-oxidation and herbivore grazing. CanJ.Fish. Aauat.Sci. 47, 1166-1176. Mantoura R.F.C. and Llewellyn CA. (1983) The rapid determination of algal chlorophyll and carotenoid pigments and their breakdown products in natural waters by reverse-phase high-performance liquid chromatography. -Anal.ChimActa, 151,297-314. Otsuki A., Takamura N. (1988) Comparison of chlorophyll-a concentrations measured by fluorometric HPLC and speetrophotometrie methods in highly eutrophic shallow Laké Kasumigaura. - Verh. Internál. Véréin. Limnol. 23,944-951. Quiblier C„ Feuillade M., Bourdier C„ Pepin D. (1994) Phytoplankton distribution as determined by HPLC pigment analisis. - Arch. Hydro biol. Beih. Ergebm. LimnolAl, 113-124. Repeta DJ. and Gagosian R.B. (1984) Transformations and recycling of carotenoids and chlorins in the Peru upwelling region (15° S, 75°W). Geochim.CosmochimActa 48, 1265-1277. Trees C.C., Kennicutt II, M.C., Brooks JM. (1985) Errors associated with the standard fluorimetric determination of chlorophylls and phaeopigments-A/orme Chemistry, 17, 1-12. Utermöhl H. (1958) Zur Vervollkommnung der quantitativen Phytoplankton Methodik. Mitt. int. theor. angew. Limnol.9, 1-38. Wilhelm C., Rudolph /., Renner IV. (1991) - A quantitative method based on HPLC-aided pigment analysis to monitor sructure and dynamics of the phytoplankton assemblage - A study from Laké Meerfelder Maar (Eifel,Germany).-Hydrobiol. 123, 21-35. Wright S. W.. Jeffrey S.W., Mantoura R.F.C.. Llewellyn CA . Bjornland T„ Repeta D.,Welschmeyer N. (1991) Improved HPLC method for the analysis of chlorophylls and carotenoids for marine phytoplankton. -Mar. Ecol. Prog. Ser. 77, 183-196. Zapala M., Ayala A., Franco JM-, Garrido J.L. (1987) Separation of chlorophylls and their degradation products in marine phytoplankton. - J. Chromatogr., 294, 281-295. Determination of phytoplankton diversity by HPLC methods Hiripi L., Kovács A. and Vörös L. Somé photosynthetic pigments like chlorophyll-a and P-carotene occur in all phytoplanton taxa, whereas certain xantophylls are specific to a few phytoplankton group. Four xantophylls, oscillaxanthin, mixoxantophyll, zeaxanthin and eichinenone, are characteristic for cyanobacteria, whereas fucoxanthin, peridinin, lutein occure predominantly in Diatomophyceae, Dinophyceae and Chlorophyceae respectively. The xantophylls can be used to determine the taxonomic composition of phytoplankton whereas the chlorophyll-a are routinely used to estimate the biomass of phytoplankton. The aim of the present study was to adapt and optimize an HPLC method to identify and measure quantitatively the pigments in algal cultures and in phytoplankton samples collected from Laké Balaton. Using a Lichrospher RP-Ci»-5p column and modification of the method of Mantoura and Llewellyn was satisfactory to separate and measure the pigments. Our results support the literature data that the biomass of the cyanobacteria can be monitored by measuring the concentration of the characteristic xantophyll of this taxa. We alsó demonstrated that not all cyanobacteria (e.g. C.raciborsku, A. flos-aque ). In such cases other xantophyll than zeaxanthin can be used to measure the biomass of cyanobacteria. cyanobacteria, pigment analysis, HPLC J_ J Tihany K1SB. HELY 2. ábra Kékalgák biomasszájának %-os aránya | sejtszcmlálással Q pigmentanalizisse!