Fogorvosi szemle, 2007 (100. évfolyam, 1-6. szám)
2007-10-01 / 5. szám
268 FOGORVOSI SZEMLE ■ 100. évf. 5. sz. 2007. 16% of the individuals involved had at least one missing tooth germ excluding wisdom teeth, and the cases with more than six missing teeth amount to more than 1% [9], These studies, which were performed according to internationally accepted standards, provide a firm basis for further molecular epidemiology investigations. This article makes an attempt towards this direction. The template for genetic studies is the DNA. Therefore, it is very important to isolate good quality, intact Table I The oligonucleotide primers and restriction endonucleases of the PCR-RFLP examinations Polymorphisms Oligonucleotide primers, temperature profile of PCR’s Product size Restriction endonuclease Size of fragments after digestion IL-1 a (-889) C/T 5’-TGTTCTACCACCTGAACTAGGC-3’ 5-TTACATATG AGCCTTCCATG -3’ 95 C° 4 min; 45 cycles: 94 °C 1 min, 50 °C 1 min, 72 °C 1 min; 72 °C 5 min 99 bp Ncol C allele: 20/79 bp, T allele: 99 bp not digested IL-1 ß (-511) C/T 5’-GCATTGATCTGGTTCATC-3’ 5’-GTTTAGGAATCTTCCCACTT-3’ 95°C 4 min, 35 cycles: 95°C, 1 min; 55°C, 1 min; 74°C, 1 min 305 bp Ddel C allele: 155/160 bp, T allele: 31/114/160 IL-1 ß (+3954) C/T 5’-CTCAGGTGTCCTCGAAGAAATCAAA-3’ 5’-GCTTTTTTGCTGTGAGTCCCG-3’ 95°C, 4 min, 35cycles: 95°C, 1 min; 59°C, 1 min; 72°C, 1 min 194 bp Taq 1 C allele: 12/73/85 bp, T allele: 12/178 bp IL-6 (-174) C/G 5’-TGACTTCAGCTTTACTCTTTGT-3’ 5’-CTGATTGGAAACCTTAT-3’ 95°C, 4 min, 35 cycles: 95°C, 45 sec; 53°C, 1 min; 72°C, 75 sec; 72 °C 5 min 198 bp Hsp 92 II C allele: 31/45/122 bp, G allele: 31/167 bp IL-10 (-1082) G/A 5’-TCTGAAGAAGTCCTGATGTCACTG-3’ 5’-ACTTTCATCTTACCTATCCCTACTTCC-3’ 95°C, 4 min, 40 cycles: 95°C, 1 min; 59°C, 1 min; 72°C, 1 min; 72°C, 1 min 196 bp Mnl I G allele: 28/58/110 bp, A allele: 58/138 bp TLR-4 (-299) G/A 5-GATTAGCATACTTAGACTACTACCTCCATG-3’ 5’-GATCAACTTCTGAAAAAGCATTCCCAC-3’ 95°C, 4 min, 30 cycles: 95°C, 30 sec; 55°C, 30 sec; 72°C, 30 sec 249 bp Nco 1 G allele: 26/223 bp, A allele: 249 bp not digested TLR-4 (-399) T/C 5’-GGTTGCTGTTCTCAAAGTGATTTTGGGAGAA-3’ 5'-ACCTGAAGACTGGAGAGTGAGTTAAATGCT-3’ 95°C, 4 min, 30 cycles: 95°C, 30 sec; 55°C, 30 sec; 72°C, 30 sec 407 bp Hinti T allele: 29/378 bp C allele: 407 bp not digested TNFa (-308) G/A 5’-AGGCAATAGGTTTTGAGGGCCAT-3’ 5’-TCCTCCCTGCTCCGATTCCG-3’ 94°C, 4 min, 35 cycles: 94°C, 2 min; 60°C, 1 min; 72°C, 1 min 107 bp Ncol G allele: 20/87 bp, A allele: 107 bp not digested PAX9( -1031) G/A 5’-AGCCTGAATCCTGTGTGCAC-3' 5’-CTAATCTAAAGTGTACCGTATGC-3’ 95°C, 3 min, 35 cycles: 95°C, 1 min; 58°C, 1 min; 72°C, 1 min 202 bp Haelll G allele: 26/176 bp, A allele:202 bp not digested PAX9( -912) C/T 202 bp Msei C allele: 45/157 bp, T allele: 13/45/144 bp
