Fogorvosi szemle, 2006 (99. évfolyam, 1-6. szám)
2006-12-01 / 6. szám
255 FOGORVOSI SZEMLE ■ 99. évf. 6. sz. 2006. may help to understand and prevent the formation of tumors and lead to new therapeutic approaches. Materials and Methods: Using Affymetryx DNA microarray and reverse transcriptase PCR, global gene expression profiles were acquired before and after serum induced differentiation from normal and SV40-T antigen immortalized human oral kératinocytes. We also investigated with immunohistochemical techniques the expression of keratins in organotypis cultures. Retinod induced differentiation and cell cycle alterations were determined by analyzing involucrin expression by western blot and by flow cytometrical analysis of propidium iodide stained cells, respectively. Results: Normal cells cultured in vitro in the absence of serum preserved the gene expression pattern characteristic for normal oral epithelial cells in vivo. Immortalization with SV40-T antigen had great impact on the expression of genes involved in the process of differentiation, extra-cellular matrix deposition and cell adhesion. Immortalization abolished the cells’ ability to differentiate upon serum exposure but increased their susceptibility towards apoptosis inducing agents. Conclusions: Based on our results we conclude that in our in vitro model cells behave similarly to normal and dysplasie oral epithelial cells in vivo. Moreover organotypic cultures of oral kératinocytes might serve as useful tools in a number of experimental systems, for instance pharmacological or toxicological investigations. THE LEGAL MILLIEU IN THE EUROPEAN UNION OF THE REGULATION OF DENTAL SUPPLIER ACTIVITY SCHMIDT, J„ JÁHN, M„ KIVOVICS, P. Department of Prosthodontics, Faculty of Dentistry, Semmelweis University, Budapest, Hungary The changes of the legal regulation in remedy seem to be very fast recently - not excluding the dental supplier activity either. Every change in the dentistry and the connection to the EU expands the legal confines of our profession. The authors wanted to show these new legal confines from the aspect of the dental supplier system. Naturally, the new legal environment is not confined only to the dental supplier system but the trends in EU initiated changes in the field of gradual and postgradual studies; new materials and standards; authorization of new instruments, etc. The determination and decision from the most important parameters in the medical practice belong to the national legislature, but the European legislation can necessitate setting up new constitutions and proceedings. There appeared a new incident, the „professional migration” across Europe after the accession of the 10 new members. Our goal is to show the new European directives and other legislatives that can prejudice of our daily routine dental work, especially the new „Professional Qualifications Directive” No. 2005/36. We want to summarize the most important professional organizations, particularly the Council of European Dentists, former DLC, and its important role in the „dental” legislation. Finally we show some of the quantitative and qualitative parameters of the European dental supplier systems. INFLUENCE OF SCOLIOTIC DEFORMATION ON DEVELOPMENT OF CRANIOFACIAL ASYMMETRIES SEGATTO, E.\ MARSCHALKÓ, P.2, VÉGH, A.1 1Heim Pál Children’s Hospital, Department of Orofacial Orthopaedics and Orthodontics, Budapest, Hungary; 2Heim Pál Children’s Hospital, Department of Orthopaedics, Budapest, Hungary Introduction: Scoliosis with a frequency of 5%o at Hungarian children is often associated with asymmetrically developed bone structures connected to the vertebral column. The aim of this study was to investigate the coherence among the direction of the different facial asymmetries, as well as among the scoliotic deformation, represented by the direction of the scoliotic main curvature. Material and methods: 52 children diagnosed with scoliosis (mean age: 15Y,0M; SD: 2Y,7M) as well as 58 children being sound in terms of orthopedics (mean age: 13Y,6M; SD: 1Y,11M) were involved in these examinations. The scoliotic children were divided into two subgroups according to the direction of the main curvature (the first with left side deviance [n=30, mean age: 15Y,6M, SD: 2Y,3M], the second with right side deviance [n=22, mean age: 14Y,1M, SD: 2Y,7M]). Characteristics of the scoliotic deformation were detected on the frontal radiograms of the vertebral column. Facial asymmetry evaluation of the P-A cephalograms has been done according to Ricketts analysis. Results: In the first phase of evaluations two maxillary asymmetry indexes were determined, which could be used for the comparisons. The final results didn’t support the presence of a significant correlation between these indexes and the direction of the main scoliotic curvature. Conclusions: Factors playing a role in pathogenesis of scoliosis have influence on the asymmetric development of the craniofacial structures of the mid-face. Asymmetrical development of these craniofacial structures cannot be derived from the direction of the main scoliotic curvature.
