Fogorvosi szemle, 2006 (99. évfolyam, 1-6. szám)
2006-12-01 / 6. szám
249 FOGORVOSI SZEMLE ■ 99. évf. 6. sz. 2006. study groups: saline controls (n=15), and VEGF (n=15) groups. Changes in gingival vessel diameter were measured by vital microscopy at the first upper incisor prior to, and 1, 5, 15, 30 and 60 minutes after VEGF (10pg/ml, 10 pi) or saline administration. For statistical analysis two-way ANOVA was applied with treatment and time as factors. Normal distribution was tested by the Kolmogorov-Smirnov test. Results: In the presence of VEGF increased vessel diameter was found in the 1st and 5th minutes (12.35 ± 3.75%; 12.66 ± 5.04%; mean + SE; p<0.05) as compared to the control group. Conclusion: These findings suggest that the immediate vasodilatation might be facilitated by VEGF receptors present in the vessels of gingiva propria. This study was supported by ETT 541/2003 and OTKA T- 42584. DNA EXAMINATION OF CHILDREN’S STREPTOCOCCUS MUTANS HERCZEGH, A.1> GHIDÁN, Á.2, DESEO, K.3, TARJÁN, I.3 department of Conservative Dentistry and Endodontics, Semmelweis University, Budapest, Hungary; department of Medical Microbiology, Semmelweis University; department of Pedodontics and Orthodontics, Semmelweis University Introduction: The pathogen capacity of microorganisms depends on their quantity, virulence and on the host’s conditions. The Streptococcus mutans (S. mutans) strains are highly heterogeneous phenotipically and genotipically. Their ability to cause disease is different. Aim of the study: The authors’ aim was to find an association between the DNA structure of S. mutans and its virulence. Materials and methods: Twenty-eight S. mutans strains from 100 children’s plaques were collected. The mean age of the children was 12,8 years. Children were divided into caries-active (DMFT >5), caries-free (DMFT=0) and gingivitis (Gl >2) groups. Mutans streptococci were selected on MSB agar. S. mutans were isolated by API rapid ID 32 Strep diagnostic system and by raffinose fermentation. Pulsedfield gel electrophoresis (PFGE) method was used for DNA structure comparison. Results: Analysing the bands of the 28 strains, 8 major PFGE pattern groups were found. Strains were closely related (defined at 70% cut off) to each other, although they were derived from plaques of three clinically different groups (DMFT > 5, DMFT=0 and Gl >2). Three pairs of identical patterns were found, originating in two cases from different groups of clinical condition. Discussion: We haven’t found correlation between the DNA structure of S. mutans and cariogenecity. The results of the PFGE experiments suggest that different DNA strains of S. mutans are not the only determining factor in the development of dental and periodontal diseases. References: 1. Keene HJ, Shklair IL: Relationship of Streptococcus mutans carrier status to the development of carious lesions in initially caries free recruits. J Dent Res 1957; 53: 1295. 2. Köhler B, Krasse B: Human strains of mutans streptococci show different cariogenic potential in the hamster model. Oral Microbial Immunol 1990; 5: 177. ANTIMICROBIAL THERAPY IN DENTAL PRACTICE HERMANN, P.1, GERA, I.2, GERLE, J.1 ROZGONYI, F.3 1 Department of Prosthodontics, Semmelweis University, Budapest, Hungary; 2 Department of Periodontology, Semmelweis University; 3 Institute of Medical Microbiology, Semmelweis University Introduction: Microbial compounds include bacterial, viral, and fungal agents even in healthy organs and all of these are prescribed in dentistry. In the dental practice protozoas have not got high importance. Aims: Antimicrobial therapy aims to treat infection with a drug to which the causative organism is sensitive. At the empirical antibiotic therapy the antimicrobials can be administered on the infectious disease, the most probable pathogen, and the usual antibiotic sensitivity pattern of the pathogen. At the rational antibiotic therapy the antimicrobial agents are administered after the sensitivity of the pathogen has been established by cultured and in vitro testing in the laboratory. Mode of action: Antimicrobial agents inhibit the growth of or kill microorganisms by a variety of mechanisms. In general the target involves the cell wall, the protein synthesis, or to interfere with nucleic acid replication. Whenever possible, a single antimicrobial agent should be used to reduce the incidence of possible side effects and emergence of resistant bacteria. In dentistry combination therapy should be avoided as far as possible. Antibacterial therapy: Amino-penicillins are the most useful and widely used antimicrobial agents in dentistry, since they are effective against a majority of Gram-positive organisms (Actinomyces, a-hemolytic streptococci, Peptostreptococci), and Gram-negative organisms such as Bakteroides, Prevotella, Porphyromonas. In dentistry 3rd generation cephalosporins should be resorted to as a second line of defense, depending on culture and antibiotic sensitivity test results.
