Fogorvosi szemle, 2005 (98. évfolyam, 1-6. szám)
2005-04-01 / 2. szám
79 FOGORVOSI SZEMLE A. RADULESCU, MC. BALOTESCU*, I. CODITA*, D. BUNGETZIANIT* RATB Health Center- Stomatology Department, *Cantacuzino Institutute, Private Homeopathy Center Bucharest, ROMANIA CLINICAL, MICROBIOLOGICAL, BIOCHEMICAL AND IMMUNOLOGICAL EVALUATION OF NEW IMMUNOSTIMULATORY METHODS IN PERIODONTAL DISEASES. STUDY DESIGN AND PRELIMINARY RESULTS Background: It is now well established that periodontal diseases are associated with resident microorganisms of the dental plaque, but their different clinical forms may also depend on different predisposing conditions such as depressed immune systems. Purposes: 1) microbiological and biochemical characterisation of periodontal specimens; 2) investigation of the correlation between host immune status and periodontal diseases; 3) optimisation of an immunostimulatory product potentially active in the treatment of periodontal diseases. Population: The study will be performed on two groups of patients with periodontal diseases; the first group will be provided with personalized, whole, bacterial suspensions of standard density obtained from cultures recovered after the cultivation of periodontal specimens on blood-agar in anaerobic conditions. The second group will be provided with whole, formaline-inactivated staphylococcal stock vaccine. In both cases, the immunostimulatory products will be included in capsules and administered sublingually. Parameters assayed before and after administration of immunostimulatory products: 1) clinical; 2) microbiological (qualitative and quantitative features of microbiota recovered fron the gingival pocket); 3) biochemical (mathrix metalloproteases level assay); 4) immunological (blood cell count, blood formula, salivary slgA assay, quantitative immunogramme, C reactive protein, immune circulant complexes and complement 2 assay. Preliminary results: Our first results indicated that: the diversity of microbiota in the analyzed specimens was extremely high among patients, but reduced in the same specimen, being represented by one or maximum two morphological types; the biochemical assay of MMP level indicated the presence of constantly high levels of MMP 9 with gelatinase activity in the pocket specimens; the immunological assays indicated high levels of IgM and C2 in patient with periodontal disease. Acknowledgement: This study was performed as part of Romanian participation in COST B23 program which kindly offerred the financial support for presentation of our results during this meeting. ■ 98. évf. 2. sz. 2005. A. ROCHAT Laboratory of Stem Cell Dynamics, Lausanne University Hospital (CHUV) and Swiss Federal Institute of Technology (EPFL) Lausanne, Switzerland STEM CELLS OF THE HAIR FOLLICULE Mammalian skin is best characterized by the presence of hair follicles, which develop during fetal life through complex epithelio-mesenchymal interactions. Hair follicles are superb model systems to study stem cell renewal, stem cell trafficking and stem cell interactions as they contain epithelial, mesenchymal and melanocyte stem cells that altogether contribute to the production of colored hairs. We have demonstrated by microsurgery that the upper region of adult hair follicles contains multipotent stem cells that can generate all the epithelial lineages of the hairy skin and form hair follicles, sebaceous gland and epidermis in response to skin morphogenetic signals (Oshima et al, Cell 2001). Skin epithelial cells can initiate colonies when cultivated under proper conditions (Rheinwald and Green, Cell 1975; Barrandon and Green, PNAS1985). Clonal analysis experiments have ' demonstrated that the multipotent epithelial stem cells of hair follicle are clonogenic, self-renew in culture, home to the proper niche when transplanted, participate to the formation of sebaceous glands and epidermis and are involved in follicle renewal for many cycles. U. SAUER , R. BURGEMEISTER PALM Microlaser Technologies AG, Am Neuland 9+12, 82347 Bernried, Germany ISOLATION AND CAPTURE OF LIVE CELLS BY LASER MICRODISSECTION AND PRESSURE CATAPULTING (LMPC) Modern molecular research relies on the capability of getting access to pure samples. Laser Microdissection and Pressure Catapulting (LMPC) is a known method to isolate and collect specific cells from complex tissues for subsequent molecular analyses. Tissue preparation and extraction protocols allow the utilization of microsamples for quantitative molecular analyses like, e.g., PCR and RT-PCR, microarrays and MALDI/SELDI spectrometry. Up to now, LMPC has been applied mostly on paraffin and cryosections, cell smears, cytospins and chromosome preparations. An important innovation is the laser driven isolation of live cells out of a cell culture. Individual cultured cells or small groups can be used for direct molecular analysis or recultivation. This helps scientists to isolate cell clones and separate different cell types by morphology or fluorescent label. The Principle of LMPC is a pulsed UV-A laser coupled into a routine research
