Fogorvosi szemle, 2005 (98. évfolyam, 1-6. szám)

2005-04-01 / 2. szám

FOGORVOSI SZEMLE ■ 98. évf. 2. sz. 2005.

77

Key words: salivary glands, adult stem cells, stress condition Acknowledgemet: Supported by COSTB23 Action

M. PILMANE, I. AKOTA*, B. BARKANE*, S. BOKA, G. NARTISA-ZAKE

Institute of Anatomy and Anthropology, ‘Institute of Stomatology, Riga Stradins University, Riga, Latvia

MORPHOFUNCTIONAL CHANGES IN PALATE SOFT AND HARD TISSUE OF CHILDREN WITH COMPLETE LIP PALETE CLEFT

Congenital facial clefts belong to the second most often malformation in newborns of the world population. Despite numerous investigations still unclear are morphopathogenetical mechanisms in development of facial clefts. The aim of this study was investigation of morphological growth and quality in the hard and soft tissue, obtained during plastical surgery from 20 children with lip palate clefts with routine, immunohistochemical and statistical methods.

Processus maxillaris showed osteones of different thickness (from 0.06±0.01 to 0.16±0.1 mm) and chaotically oriented osteocytes. Number of cells in bone varied from 192.397±33.3 to 439.469+52.2 per mm2. Intensive staining for substance P was seen in osteocytes. However, chaotically organized cells contained week immunorectivity for peptide or even absence of it. Apoptosis was not detected in bone. Hyaline cartilage of spina nasalis anterior showed condensation of chromatin in some chondroblasts and chondrocytes and almost all cells were positive for substance P. Number of cells in cartilage varied from 32.24±21.91 to 174.3±69.16. TUNEL discovered slightly positive nuclei of many chondrocytes.

Palate epithelium showed hyperplasia, atrophy or branched epithelial cords in the underlaying connective tissue. Also regional vacuolization of basal cells was detected. Inflammation with epitheloid cells, plasma cells, white blood cells, and prominent nerve bundles, sclerotic blood vessels and neoangiogenesis was seen in subepithelium. Apoptosis affected muscle and connective tissue.

Conclusions. Hard tissue in children with lip palate cleft possessed degeneration and beginning of apoptosis in hyaline cartilage of spina nasalis anterior, and unregular growth of processus maxillaris. Degenerative changes of bone relate to chaotic localization of collagen, development of prominent osteones with limited number of chaotically placed cells without apoptosis. Main changes in soft tissue include the atrophy, hyperplasia, growth of epithelium in the connective tissue, neoangiogenesis and inflammation, suggesting about regeneration variations of tissue. Acknowledgement: The support of COSTB23 is gratefully acknowledged.

I.BARKANA, S. A. NARAYANAN*, N. SAVION, S. PITARU

Tel Aviv University, Israel, ‘Washington University, Seattle, USA

CEMENTUM ATTACHMENT PROTEIN (CAP) INDUCES CEMENTO-BLASTIC DIFFERENTIATION IN PRIMITIVE PROGENITORS OF HUMAN PERIODONTAL LIGAMENT

The adult human periodontal tissues originate from two main cell lineages: a fibroblastic lineage (Fb) and a mineralized tissue-forming lineage comprising the cementoblastic (Cb) and osteoblastic (Ob) subsets. CAP is a chimerical protein consisting of PTPLA possibly bound to collagen (Narayanan et al In preparation). Utilizing a cell cloning approach and CAP binding and expression i we isolated and established 3 cohorts of clones representing primitive progenitors of these lineages.

The purpose of the present study was to test whether CAP does act as a differentiation factor for the cementoblastic lineage.

Cultures of Fb, Ob, and Cb primitive cloned progenitors and of alveolar bone cells (ABC) and of a cementoma cell line (CC) grown in the presence of Dex, vit C and ß-glycerophosphate were treated with CAP. Untreated cultures served as controls. CAP induced the expression of BSP in otherwise BSP negative Fb progenitors and increased BSP expression in all cultures. CAP induced [ alkaline phosphatase expression and mineralized tissue formation in Fb progenitors; it increased mineralized tissue formation in Cb and CC cultures but reduced considerably this parameter in Ob and ABC cultures. In contrast to the characteristic spread out nodular pattern of mineralized tissue formation observed in Ob and ABC cultures, the mineralized tissue formed in Cb and CC cultures exhibited a well defined ridge-like seam configuration. Treatment of ABC and Ob cultures with CAP changed the pattern of mineralization from the nodular (osteoblastic) configuration to the ridge-like seam (cementoblastic) one.

These results indicate that CAP is a putative differentiation factor for the cementoblastic lineage and suggest for the first time that the Cb, Fb and Ob cell lineages of the adult human PDL originate from a common multipotential progenitor population.

Key words: cementum, attachment protein, cementoblastic progenitors Acknowledgement: The study was supported by an ISF grant and its presentation by COST Action B23.