Fogorvosi szemle, 2005 (98. évfolyam, 1-6. szám)
2005-04-01 / 2. szám
70 FOGORVOSI SZEMLE ■ 98. évf. 2. sz. 2005. the “bone paste” for grafting. Bone cores were extracted from patients respectively sixth months and ten months after grafting. The cores were formalin-fixed, decalcified, paraffin embedded, cut, Hematoxylin/Eosin and/or immunohistochemically stained using antibodies against either pro-inflammatory proteins (S100A8 and S100A9 proteins) (Thorey IS. (2001) J Biol Chem, 276:35818-25) or S100B, in order to characterise the tissues. After sixth months grafted cores, histology revealed a high integration of the PepGen P-15 in the fibrous tissue, the presence within a large area of regenerated natural bone and PepGen P-15 within the new reconstructed bone, a surrounding of those tissues by osteoblasts and a densely vascularised and highly organised connective tissue. S100A8/A9 immunohistochemistry revealed very few activated neutrophils and macrophages in the connective tissue, indicating no infection in the graft. S100B immunohistochemistry revealed the colonization of both PepGen P-15 and natural bone by osteocytes. In the ten months grafted bone cores, we observed that, firstly, the quantity of PepGen P-15 considerably decreased to become natural bone and, secondly, the connective tissue was less fibrous and became scattered with adipocytes. Immunohistochemistry also revealed that antibodies against S100A8 and S100A9 might serve as PepGen P-15 markers. Keywords: tissue factor, PRP, sinus lift. Acknowledgments: V.Daubie is a fellow of a FIRST EUROPE grant. Supported by COST B23. A. GRUART, M. STREPPEL*, O. GUNTINASLICHIUS*, D.N. ANGELOV**, W.F. NEISS**, J.M. DELGADO-GARCÍA Division de Neurociencias, Universidad Pablo de Olavide, Sevilla, Spain. ‘Klinik für Hals-, Nasen- und Ohrenheilkunde, and ‘‘Institut I für Anatomie der Universität zu Köln, Köln, Germany. MOTONEURON ADAPTABILITY TO NEW MOTOR TASKS FOLLOWING DIFFERENT TYPES OF FACIALFACIAL ANASTOMOSIS IN ALERT BEHAVING CATS The ability of the facial motor system to adapt to a new motor function was studied in alert behaving cats after unilateral transection, 180 deg rotation and suture of the zygomatic nerve, or transection and cross anastomosis of the proximal stump of the buccal nerve to the distal stump of the zygomatic nerve. These procedures induced reinnervation of the orbicularis oculi (OO) muscle by different 00- or mouth-related facial motoneurons, respectively. Eyelid movements and the electromyographic activity of the OO muscle were recorded up to one year following the two types of anastomosis. Animals with a zygomatic nerve rotation recovered spontaneous and reflex responses, but with evident deficits in eyelid kinematics; that is, the proper regional distribution of OO motor units was disorganized by zygomatic nerve rotation and resuture, producing a permanent defect on eyelid motor performance. Following buccal-zygomatic anastomosis, the electrical activity of the OO muscle was recovered after 6-7 weeks, but air puff-, flash-, and toneevoked reflex blinks never reached the control values on the operated side. Electromyographic OO activities and lid movements corresponding to licking and deglutition activities were observed on the operated side in buccal-zygomatic anastomosed animals up to one year following surgery. Mouth-related facial motoneurons did not readapt their discharges to the kinetic, timing, and oscillatory properties of OO muscle fibers. A significant hyperreflexia was observed following both types of nerve repair in response to air puffs, but not to light flashes or tones. In conclusion, adult mammal facial premotor circuits maintain their motor programs when motoneurons are induced to reinnervate a foreign muscle, or even a new set of muscle fibers. Aknowledgemet: Supported by DGICYT PM98-011 in Spain, the Jean Uhrmacher Foundation in Germany, and COST Action B23. Y LEISER, L DAFNI, A.L. TAYLOR, E ROSENFELD, B SHAY, E1. FERMON, A HAZE-FILDERMAN, Y COHEN, A BOOM**, R POCHET“, E SHAI, A BLUMENFELD, R TABAKMAN“, P LAZAROVICI*, D.D. DEUTSCH Dental Research Laboratory, The Institute of Dental Sciences Faculty of Dental Medicine, ‘Department of Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew University-Hadassah, Jerusalem, Israel “Laboratory of Histology, Neuroanatomy and Neuropathology, Faculté de Médecine, Université Libre de Bruxelles, Bruxelles, Belgium. THE INDUCTION OF THE TUFTELIN GENE IN-VIVO IN THE MOUSE BRAIN AND IN-VITRO IN THE NEURONAL MODEL- PC12 CELL LINE DURING HYPOXIA The Tuftelin gene is expressed at very early stages of tooth bud development. It is thought to play an important role during the development and mineralization of enamel. Our search of the EST database and our previous studies have shown that the tuftelin mRNA and protein wereare also expressed in various normal and cancerous soft tissues such as embryonic morula, brain etc., suggesting that tuftelin may have a universal or multifunctional role. In the mouse brain tuftelin was specifically expressed in the neurons, was differentially expressed at different regions of the brain, and the levels of tuftelin mRNA expression in the brain increases significantly with age from prenatal to seven month old mice (Deutsch et al 2002a,b; Leiser et al 2002, 2004). Tuftelin was
