Fogorvosi szemle, 2005 (98. évfolyam, 1-6. szám)

2005-12-01 / 6. szám

250 FOGORVOSI SZEMLE ■ 98. évf. 6. sz. 2005. mtsai [13] vizsgálatukban leírtak. A továbbiakban ter­vezzük a végbement változások reverzibilitásának, a flu­­oridok hatásának, illetve az időfaktor szerepének vizs­gálatát. Ismeretes ugyanis, hogy a fogfehérítés során jelentkező fogérzékenység kezelésére leggyakrabban fluoridokat alkalmaznak. Ezek feltehetően a degradált apatit felületéhez képesek kötődni, illetve a károsodott organikus mátrixon keresztül a zománc mélyebb rétege­ibe is eljuthatnak. A hosszabb kezelési idő ugyanakkor feltehetően növeli a változásokat. Irodalom 1. Anderson DG, Chiego DJ, Glickman GN, McCauley LK: A clinical assessment of the effects of 10% carbamide peroxide gel on human pulp tissue. J Endod 1999; 25: 247-250. 2. Arends J, Jongebloed WL, Goldberg M, Schuthof J: Interaction of urea and human enamel. Caries Res 1984; 18:17-24. 3. ArwillT, Myrberg N, Söremark R: Penetration of radioactive iso­topes through enamel and dentine II. Transfer of 22Na in fresh and chemically treated dental tissues. Ódont Revy 1969; 20: 47-54. 4. Attin T, Kielbassa AM, Schwanenberg M, Hellwig E: Effect of fluo­ride treatment on remineralization of bleached enamel. J Oral Rehabil 1997; 24: 282-286. 5. Attin T, Hanning C, Wiegand A, Attin R: Effects of bleaching on restorative materials and restorations - a systematic review. Den Mat 2004; 20: 852-861. 6. Avery JK: Histology of Enamel. In: Oral Development and Histology. Thieme Medical Publishers Inc., New York, 1994; 231-233. 7. Bitter NC: A scanning electron microscopy study of the effect of bleaching agents on enamel: A preliminary report. J Prosthet Dent 1992; 67: 852-855. 8. Boyde A: Microstructure of enamel. In: Dental Enamel. Wiley, Chi­chester (Ciba Foundation Symposium 205) 1997; 18-31. 9. Cohen SC: Human pulpal response to bleaching procedures. J Endod 1979; 5: 134-138. 10. Cole AS, Eastoe JE: Chapter 32. Enamel. In: Biochemistry and Cell Biology. Wright, Bristol, 1988; 461-463. 11. Ernst CP, Marroquin BB, Willershausen-Zönnchen B: Effects of hydrogen peroxide-containing bleaching agents on the morphology of human enamel. Quint /nM996; 27: 53-56. 12. Goldberg M, Arends J, Jongebloed WL, Schuthof J, Septier D: Action of urea solutions on human enamel surfaces. Caries Res 1983; 17: 106-112. 13. Haywood VB, Heymann HO: Nightguard vital bleaching. Quint Int 1989; 20: 173-176. 14. Hegedűs C, Bistey T, Flora-Nagy E, Keszthelyi G, Jenei A: An atomic force microscopy study on the effect of bleaching agents on enamel surface. J Dent 1999; 27: 509-515. 15. Janoff LE: Origin and development of hydrogen peroxide disin­fection systems. CLAO Journal. 1990; 16:1. 16. McCracken MS, Haywood VB: Demineralization effects of 10 per­cent carbamide peroxide. J Dent 1996; 24: 395-398. 17. McGuckin RS, Babin JF, Meyer BJ: Alterations in human enamel surface morphology following vital bleaching. J Prosthet DenM992; 68: 754-760. 18. Murchison DF, Charlton DG, Moore BK: Carbamide peroxide bleaching: Effects on enamel surface hardness and bonding. Oper DenM992; 17: 181-185. 19. Riddles PW, Blakeley RL, Zerner B: Reassessment of Ellman’s reagent. Method Enzymol 1983; 91: 49-60. 20. Robertson WD, Melfi RC: Pulpal response to vital bleaching pro­cedures. J Endod'\ 980; 6: 645-649. 21. Rotstein I, Danker E, Goldman A, Heling I, Stabholz A, Zalkind M: Histochemical analysis of dental hard tissues following bleaching. J Endod 1996;22:23-26. 22.Sasaki T, Takagi M, Yanagisawa T: Structure and function of sec­retory ameloblasts in enamel formation. In: Dental Enamel. Wiley, Chichester (Ciba Foundation Symposium 205) 1997; 32-50. 23. Seal NS, Wilson CF: Pulpal response to bleaching of teeth in dogs. PediatrDent 1985; 7: 209-214. 24.Seal NS, McIntosh JE, Taylor AN: Pulpal reaction to bleaching of teeth in dogs. J Dent Res 1981 ; 60: 948-953. 25. Seghi RR, Denry I : Effect of external bleaching on the indentation and abrasion characteristics of human enamel in vitro. J Dent Res 1992; 71: 1340-1344. 26.Shannon H, Spencer P, Gross K, Tira D: Characterisation of enamel exposed to 10% carbamide peroxide bleaching agents. Quint /nf 1993; 24: 39-44. 27. Titley KC, Torneck CD, Smith DC: The effects of concentrated hydrogen peroxide solution on the surface morphology of human tooth enamel. J Endod 1988; 14: 69-74. 28. Tong LSM, Pang MKM, Mok NYC, King NM, Wei SHY: The effects of etching, micro-abrasion, and bleaching on surface enamel. J Dent Res 1993; 72: 67-71. 29. Van der Werf KO, Putman CAJ, De Grooth BG, Segerink FB, Schipper EH, Van Hülst NF, Greve J: Compact stand-alone atomic force microscope. Rev Sei Instrum 1993; 64: 2892-2897. 30. WanderaA, Feigal RJ, DuoglasWH, Pintado MR: Home-use tooth bleaching agents: An in vitro study on quantitative effects on enamel, dentin, and cementum. Quint Int 1994; 25: 541-546. 31. Wright TJ, Hall K, Yamauchi M: The protein composition of nor­mal and developmentally defective enamel. In: Dental Enamel. Wiley, Chichester (Ciba Foundation Symposium 205) 1997; 85-97. Bistey T, Jenei A, Szondy Zs, Nagy Pl, Hegedűs Cs: In vitro study of the effects of 10-30 % hydrogen peroxide solutions on the human teeth’s enamel surface The aim of the present study was to investigate the effects of hydrogen peroxide solutions (10-30%) on the enamel sur­face and the inner structure of human teeth. Prepared enamel samples were exposed to various concentrations of hyd­rogen peroxide solutions for one hour. Changes in the enamel were analized by two different methods. The inner struc­ture was studied by the Ellman’s reagent to detect possible changes in the amount of free thiol groups in the remaining organic phase of matured enamel. Surface alterations were imaged by atomic force microscope. Ellman’s reaction revealed the presence of three groups of free thiols: one with easy, the second one with medium and the third one with difficult accessibility by hydrogen peroxide. Atomic force microscopy revealed severe alterations on the enamel surface after treatment with both low and high concentration of hydrogen peroxide. Our observations indicated that hydrogen peroxide induced alterations in the surface structure and in the inner phases of mature enamel in both low and high concentrations. Key words: tooth bleaching, hydrogen peroxide, atomic force microscopy, organic enamel phase, inorganic enamel phase

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